BIOSYNTHESIS OF POLY-?-HYDROXYBUTYRATE AND DISTRIBUTION OF phbC GENE IN LACTOBACILLUS PLANTARUM.

  • Department of Biomedical Engineering, VMKV Engineering College, Vinayaka Missions University, Salem-636308. India.
  • PG and Research Department of Biotechnology, Kongunadu Arts and Science College, Coimbatore-641029. India.
  • Department of Microbiology, Nehru Arts and Science College, Coimbatore-641105. India.
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Accumulation of synthetic plastics in the environment has become a worldwide problem. Polyhydroxy alkanoates (PHA) are biodegradable plastics, an alternative to petroleum-based synthetic plastics. In this investigation, the best known PHA, poly-?-hydroxybutyrate (PHB) accumulating strains of Lactobacillus plantarum were isolated from various food and environmental samples using MRS agar medium. Among the 31 isolates, 22 strains were identified as Lactobacilli by comparing the biochemical profile with Lactobacillus plantarum MTCC6160. The L. plantarum strains were confirmed with 16S rRNA identification method and 11 among the 22 Lactobacillus strains were revealed the characteristic band at 735 bp. The partially amplified product was sequenced and the comparison of the sequence in NCBI BLAST obtained 94% similarity with the strain, L. plantarum IMAU70089. The isolated strains of L. plantarumwas subjected to PHB production using nitrogen limited minimal medium (NLMM).The inclusion bodies were stained with Sudan Black B and the organism was also plated on Nile blue A medium, revealed the presence of a lipid material, which was confirmed as PHB by the analysis of molecular fragments by FTIR spectroscopy in comparison with reference material. In addition, the existence of phbC gene (578 bp) was identified, which encodes the enzyme, PHB synthase for accumulating PHB. This investigation confirmed that 1 mg of the cell dry weight constitutes the maximum of 40.4 µg of PHB. This study revealed that L. plantarumhas been recognized as a good candidate for PHB.

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K. Natarajan, R. Subashkumar, Minu Venugopal and J. Rathinamala (2017); BIOSYNTHESIS OF POLY-?-HYDROXYBUTYRATE AND DISTRIBUTION OF phbC GENE IN LACTOBACILLUS PLANTARUM., Int. J. of Adv. Res., 5 (02), 1847-1855, ISSN 2320-5407. DOI URL: https://dx.doi.org/10.21474/IJAR01/3350

K. Natarajan
VMKV Engineering College, Vinayaka Missions University, Salem-636308. India.

DOI:

Article DOI: 10.21474/IJAR01/3350      
DOI URL: https://dx.doi.org/10.21474/IJAR01/3350

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