25Mar 2017

PHYTOCHEMICAL SCREENING, ANTIOXIDANT & ANTIBACTERIAL ACTIVITY OF GREEN SYNTHESIZED SILVER & GOLD NANOPARTICLES USING LEAF EXTRACT OF ZIZIPHUS NUMMULARIA AND ITS EFFECTIVENESS AGAINST INFECTED DENTINE: AN IN VITRO STUDY.

  • Department of Biotechnology, Maharaj Vinayak Global University, Dhand, Tehsil- Amer 302028, Jaipur, Rajasthan.
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Green synthesis is a promising nanotechnological tool for biomedical application. The present investigation demonstrates a rapid biogenic approach for the synthesis of gold and silver nanoparticles using biologically active and medicinal important Ziziphus nummularia leaf extract as a reducing and stabilizing agent under ambient conditions. To our knowledge, this is the first report where Zizyphus nummularia leaf extract was found to be an appropriate plant source for the green synthesis. The presence of various phytochemicals viz. polyphenols, alkaloids and flavonoids, were investigated by following standard biochemical methods. The silver and gold nanoparticles were characterized by UV-visible spectroscopy (Systonic 2203) that showed Surface Plasmon Resonance (SPR) for both gold and silver nanoparticles at 551 and 438?nm. Scanning electron microscopy (SEM) revealed that gold nanoparticles were observed as nanorods in shape with 54 nm in size while the size of the silver nanoparticles was measured 200 nm with spherical shape. The antioxidant activity was determined by 2, 2- diphenylpicrylhydrazyl (DPPH) assay. Further, the antibacterial activity of synthesized silver and gold nanoparticles showed effective inhibitory activity against Staphylococcus aureus and Sstreptococcus mutans.

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[Kamini Parmar and O.P. Jangir. (2017); PHYTOCHEMICAL SCREENING, ANTIOXIDANT & ANTIBACTERIAL ACTIVITY OF GREEN SYNTHESIZED SILVER & GOLD NANOPARTICLES USING LEAF EXTRACT OF ZIZIPHUS NUMMULARIA AND ITS EFFECTIVENESS AGAINST INFECTED DENTINE: AN IN VITRO STUDY. Int. J. of Adv. Res. 5 (Mar). 664-672] (ISSN 2320-5407). www.journalijar.com

Kamini Parmar
Department of Biotechnology, Maharaj Vinayak Global University Dhand, Tehsil- Amer 302028, Jaipur, Rajasthan

DOI:

Article DOI: 10.21474/IJAR01/3556      
DOI URL: https://dx.doi.org/10.21474/IJAR01/3556

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