27Mar 2017

PHYTOCHEMICAL SCREENING, ACUTE TOXICITY AND CARDIOVASCULAR EFFECTS OF WATER EXTRACT OF HALLEA LEDERMANNII (K. KRAUSE) VERDC (RUBIACEAE), MEDICINAL PLANT FROM GABON.

  • Laboratory of Naturel Substances and Organometallic Synthesis, University of Sciences and Technology of Masuku, BP 943, Franceville-Gabon.
  • Chemistry Unit of Plant and Life, Faculty of Science and Technology, University Marien Ngouabi, BP 69, Brazzaville-Congo.
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Hallea ledermannii is a medicinal plant commonly used in traditional Gabonese medicine to fight against high blood pressure. The objective of this study was to determine the chemical profile and evaluate the acute toxicity and effect of the aqueous extract of Hallea ledermannii on cardiovascular parameters in normotensive rats. Phytochemical screening was carried out by using the usual methods of Bouquet. Acute oral toxicity was evaluated in mice at doses of 50, 300, 2000 and 5000 mg / kg in accordance with OECD Guideline 423. Tests on blood pressure and heart rate were realized by the invasive method in normotensive rats anesthetized at urethane 15%. The results show that Hallea ledermannii contains many secondary metabolites. Its polar extracts have of the low contents in total phenol (3.55675 ? 0.083, 3.27425 ? 0.101, 2.78675 ? 0.110 mg GAE/g of drugs) and total flavonoids (1.2272 ? 0.197; 0.7232 ? 0.187; 0.8072 ? 0.129 mg QE/g of drugs). Aqueous extract of Hallea ledermannii is not toxic up to the 5000 mg/kg dose. This extract (10, 20 and 30 mg/kg) causes a low in systolic blood pressure and heart rate. These results suggest that the aqueous extract of Hallea ledermannii can be used orally up to the 5000 mg / kg dose without risk of toxicity and has chronotropic negative and hypotensive effects that could explain its use in traditional Gabonese medicine against high blood pressure.

  1. Adeleke Adesegun., Anyika, N.E., OluseyiAdekoya, T. and Essien, S. G. (2012). Antibacterial and antioxidant investigations of Hallea ledermannii leaf extract. Indian Journal of Science and Technology.5 (1): 1885 -1887.
  2. BIDIE, Alain dit Philippe., N?GUESSAN, Banga B., YAPO, Adou F., N?G UESSAN, Jean David. et DJAMAN, Allico Joseph. (2011): Activit?s antioxydantes de dix plantes m?dicinales de la Pharmacop?e ivoirienne. Sciences & Nature, Vol. 8 N?1: 1-11
  3. Bouquet (1972): Plantes m?dicinales du Congo Brazzaville. O.R.S.T.O.M; Paris 13: p 8-13; 30.
  4. Bruneton, J. (1999). ?? In:?? Pharmacognosie,?? phytochimie,?? Plantes m?dicinales, TEC&DOC (Ed.), Paris, pp. 369-404.
  5. Burkill, H.M. (1997): Useful plants of West Tropical Africa; Royal Botanic Gardens; Key; Vol.4; 970p.
  6. Dimo T., Nguelefact T. B., Tan P. V., Yewah M. P. Dongo E., Rakotonirina S. V., Kamany A. I. and Bopelet M. (2003): Possible mecanisms of action of the neutral extract from Biden spilosa leaves on the cardiovascular system of anaesthetized rats. Pyrother Research., 17: 1135-1139.
  7. EtouOssibi A.W. (2010): Effets cardiovasculaires et antioxydants des extraits aqueux et hydro?thanolique de LippiamultifloraMoldenke (verbenaceae). Thèse de Doctorat unique, Universit? Marien Ngouabi, R?publique du Congo; 198 p.
  8. EtouOssibi A.W., Elion Itou R.D.G.,Nzonzi J., et al., (2014): Effets de l?extrait aqueux de LippiamultifloraMoldenke(Verbenaceae) sur la pression art?rielle, la fr?quence cardiaque et les ondes de l??lectrocardiogramme chez le rat normotendu. Revue CAMES-S?rie Pharm. M?d. Trad. Afr. 17(1): 1-9.
  9. Etou Ossibi A.W., Elion Itou R.D.G., Nkounkou Loupangou C., HibandzaNyamibara J.D., BonazabaMilandou L.J.C., Ouamba J.M., Abena A.A. (2016): Effets des extraits polaires des feuilles de Tremaorientalis (Linn.) Blume (Ulmaceae) sur la pression art?rielle moyenne chez le rat; 18(1): 8-15
  10. Falleh, H.,Ksouri, R., Chaieb, K., Karray-Bouraoui, N., Trabelsi, N., Boulaaba, M., Abdelly, C. (2008): Phenolic composition of Cynaracardunculus L. organs, and their biological C. R. Biologies; 331: 372-379.
  11. Gassita J.N., NzeEkekang Lucienne., De Vichy H?lène., Mels Louis Adrian., Koudogbo Blaise., Ekomie Richard. (1982): Les Plantes M?dicinales du Gabon; Mission Ethnobotanique de l?ACCT au Gabon; Libreville; 55p.
  12. Guyton A. C. (1989): Trait? de physiologie m?dicale. 1ère?dition, 3e 1105: 205-208.
  13. Karou, D.,Savadogo, A., Canini, A., Yameogo, S., Montesano, C., Simpore, J., Colizzi, V. and Traore, A.S. (2005): Antibacterial activity of alkaloids from Sidaacuta. Afr. J. of Biotechnol., 4:1452-1457.
  14. Kearney PM, Whelton M, Reynolds K, Whelton PK, He J. (2004): Worldwide prevalence of hypertension: a systematic review. J Hypertens. 22(1):21-4.
  15. Kim, D.O., Chun, O.K., Kim, Y.J., Moon, H.Y. et Lee, C.Y. (2003): Quantification of polyphenols and their antioxidant capacity in fresh plums. Journal of Agriculture and Food Chemistry, 51(22): 6509 6515.
  16. Martin, S. etAndriantsitohaina, R. (2002): M?canismes de la protection cardiaque et vasculaire des polyph?nols au niveau de l?endoth?lium. Annales de cardiologieetd?ang?iologie; 51:304-315.
  17. Mibindzou Mouellet, A. (2004): Screening phytochimique des deux espèces de plantes: Crotaliaretusa (Papilionaceae) et Hallea ciliata Aubrev et Pellegr (Rubiaceae) r?colt?es au Gabon. Thèse de Doctorat, Universit? de Bamako, p.88
  18. Nguelefact T. B. (2008): Effets analg?siques et cardiovasculaires des extraits de feuilles de Kalanchoecreneta (Andrews) Hawort. Thèse de Doctorat PhD, Universit? de Yaound? I - Cameroun, p.162.
  19. (2001): Ligne directive de l?OECD pour les essais de produits chimiques: toxicit? orale aigue-m?thode par classe de toxicit? aig?e; N?423, p.14
  20. (1998): Harmonized Integrated Hazard Classification System for Human Health and Environmental Effects of Chemical Substances as endorsed by the 28th Joint Meeting of the Chemicals Committee and the Working Party on Chemicals in November 1998, Part 2, p.11.
  21. Organisation Mondiale de la Sant?. (2002): Strat?gie de l?OMS pour la M?decine Traditionnelle; Organisation Mondiale de la Sant??; Genève; 65p.
  22. Raponda-Walker, A. et Sillans, R. (1961): Les Plantes Utilis?es au Gabon; Paul Lechevalier; Paris, 616p.
  23. Singleton, V.L.et Rossi, J.A. (1965): Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of technology and Viticulture, 16: 144-153.
  24. Sivananthan, M. and Elamaran, M. (2013): In vitro evaluation of antibacterial activity of chloroform extract Andrographispaniculata leaves and roots, Duriozibethinus wood bark and Psidium guajava leaves against selected bacterial strains., 3: 12-19.
  25. Souleymane F. (2004): Exploration Biochimique sur le Pouvoir Immunogène de trois Plantes en Côte d?Ivoire: Alstonia boonei (Apocynaceae), Mitragyna Ciliata (Rubiaceae) et Terminalia catappa (Combretaceae). Thèse de Doctorat, Universit? de Bamako., p.123.
  26. Svoboda, K.P&. Hampson, J.B. (1999): Bioactivity of essential oil of selected temperate aromatic plants: antibacterial, antioxidant, anti-inflammatory and other related pharmacological activities. Ed: Plant Biology Department, SAC Auchincruive, Ayr, Scotland, Uk., KA6 5HW.
  27. Vyas, N., Tailan, M.,Gavatia, N.P. et Gupta, B.K. (2010): Antioxidant potential of Psidium guajava Linn. Int. J. of Pharm. Tech. Res., 2: 417-419.
  28. Wagner Alain. (1986): Aspects des M?decines Traditionnelles du Gabon; Editions universelles; Toulouse., 332p.
  29. Xu, Y.C., Leung, S.W.S., Yeung, D.K.Y., Hu, L.H., Chen, G.H., Che, C.M., Man, R.Y.K. (2007): Structure- activity relationships of flavonoids for vascular relaxation in porcine coronary artery. Phytochemistry., 68: 1179-1188.

[Mba Akue Rony, Bikanga Raphael, Etou Ossibi Arnaud Wilfrid, Nkounkou Loumpangou Celestine and Ouamba Jean-Maurille. (2017); PHYTOCHEMICAL SCREENING, ACUTE TOXICITY AND CARDIOVASCULAR EFFECTS OF WATER EXTRACT OF HALLEA LEDERMANNII (K. KRAUSE) VERDC (RUBIACEAE), MEDICINAL PLANT FROM GABON. Int. J. of Adv. Res. 5 (Mar). 1055-1063] (ISSN 2320-5407). www.journalijar.com

MBA AKUE Rony
1. Laboratory of Naturel Substances and Organometallic Synthesis, University of Sciences and Technology of Masuku, BP 943, Franceville-Gabon

DOI:

Article DOI: 10.21474/IJAR01/3605      
DOI URL: https://dx.doi.org/10.21474/IJAR01/3605

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