An amperometric approach towards construction of a cholesterol biosensor

The cholesterol oxidase enzyme breaks down cholesterol into an intermediate cholest-5-en-3-one, which is then converted to cholest-4-en-3-one (Smith and Brooks 1997) . In the present study this mechanism was exploited. The current produced by the release of electrons from the above reaction was used to determine the amount of free cholesterol in the serum sample. The functioning of this amperometric biosensor was checked over a temperature ranging from 20?C to 60?C and a pH range of 5 to 9.