GASTROPROTECTIVE EFFECT OF A NEW FORMULATED MILK TABLET ON ETHANOL-INDUCED GASTRIC MUCOSAL INJURY IN RATS.

  • Zoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
  • Dairy Technology Department, Animal Production Research Institute, Agricultural Research Center, Giza, Egypt.
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FM is a newly formulated milk tablet that provides the supplementary food such as milk and honey, also fortified it with high nutritive value components (bee pollen, palm pollen, Royal jelly, Propolis, Ginseng,and Bee pollen) that have many remarkable biological activities such as antioxidant, antibacterial and anti-inflammatory features.The current study aimed to investigate the potential gastroprotective effects of FM against ethanol-induced gastric injury in male albino rats. The experimental set included four groups; Control group, an ulcer control group treated with absolute ethanol (10 ml/kg), FM pretreated groups at doses (157 and 314 mg/kg per oral). The gastric secretion was collected for assessment of pH. The involvement of malondialdehyde (MDA), catalase (CAT), nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α),alanine aminotransferase(ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), Uric acid, and creatinine were also measured. Histopathological studies were undertaken to evaluate the influence of the treatment on the gastric wall mucosal layer. FM suppressed gastric inflammation induced as a result of ethanol administration by decreasing of tumor necrosis factor-α (TNF-α), nitric oxide (NO) and neutrophil infiltration. Meanwhile, FM halted gastric oxidative stress via reduction of MDA level with concomitant enhancement of the activity of a potent antioxidant CAT. These favorable actions were associated with attenuation of the severity of ethanol gastric mucosal injury as evidenced by the morphological and histological observations. Together, these findings postulated the potent gastroprotective action of FMagainst ethanol-induced gastric injurywhichwas mediated via suppression of gastric inflammation and oxidative stress besides boosting of the antioxidant defense.

  1. Brown DM, Everhart JE. Cost of digestive diseases in the United States. In Digestive diseases in the United States: epidemiology and impact. Everhart JE. Washington, DC. National Institutes of Health Publication 1994; 57?82.
  2. Sandler RS, Everhart JE, Donowitz M, Adams E, Cronin K, Goodman C, Gemmen E, Shah S, Avdic A, Rubin R. The burden of selected digestive diseases in the United States. Gastroenterol 2002; 122: 1500?1511.
  3. Calam J, Baron JH. Pathophysiology of duodenal and gastric ulcer and gastric cancer. Brit Med J 2001; 323:980?983.
  4. Nemeroff CB, Hernandez DE, Orlando RC, Prange AJ. Cytoprotective effect of centrally administered neurotensin on stress-induced gastric ulcers. Am J Physiol 1982; 242:G342?346.
  5. Franke A, Teyssen S, Singer MV. Alcohol-related diseases of the esophagus and stomach. Dig Dis 2005; 23 (3?4):204?213.
  6. Al Batran R, Al-Bayaty F, Jamil AMM, Abdualkader AM, Hadi HA, Ali HM, Abdulla MA. In vivo antioxidant and antiulcer activity of Parkiaspeciosaethanolic leaf extract against ethanol-induced gastric ulcer in rats. PLoS One 2013;8 (5):e64751.
  7. Mei X, Xu D, Xu S, Zheng Y. Novel role of Zn(II)-curcumin in enhancing cell proliferation and adjusting proinflammatory cytokine-mediated oxidative damage of ethanol-induced acute gastric ulcers. ChemBiol Interact. 2012; 197(1):31?39.
  8. Sangiovanni E, Vrhovsek U, Rossoni G, Colombo E, Brunelli C, Brembati L, Trivulzio S, Gasperotti M, Mattivi F, Bosisio E, Dell'Agli M. Ellagitannins from Rubus berries for the control of gastric inflammation: in vitro and in vivo studies. PLoS One 2013; 8(8), e71762.
  9. Loguercio C, Taranto D, Beneduce F, Vecchio BC, Vincentilis A, Nardi G, Glutathione prevents ethanol-induced gastric mucosal damage and depletion of sulfhydryl compounds in humans. Gut 1993; 34:161-5.
  10. Liu Y, Tian X, Gou L, Fu X, Li S, Lan N, Yin X.Protective effect of l-citrulline against ethanol-induced gastric ulcer in rats. Environ ToxicolPharmacol 2012;34(2): 280?287.
  11. Salga MS, Ali HM, Abdulla MA, Abdelwahab SI. Gastroprotective activity and mechanism of novel dichlorido-zinc(II)-4-(2-(5-methoxybenzylideneamino)ethyl) piperazin-1-iumphenolat e complex on ethanol- induced gastric ulceration. ChemBiol Interact 2012; 195(2):144?153.
  12. Halabi MF, Shakir RM, Bardi DA, Al-Wajeeh NS, Ablat A, Hassandarvish P, Hajrezaie M, Norazit A, Abdulla MA. Gastroprotective activity of ethyl-4-((3,5-di-tert-butyl-2-hydroxy benzy- lidene) amino)benzoate against ethanol-induced gastric mucosal ulcer in rats. PLoS One 2014; 9(5):e95908.
  13. Antonisamy P, Subash-Babu P, Alshatwi AA, Aravinthan A, Ignacimuthu S, Choi KC, etal. Gastroprotective effect of nymphayol isolated from Nymphaeastellata (Willd.) flowers: Contribution of antioxidant, anti-inflammatory and anti-apoptotic activities. ChemBiol Interact 2014; 224: 157?163.
  14. Livney, Y.D., 2010. Milk proteins as vehicles for bioactives, Current Opinion in Colloid & Interface. Science. 15, 73-83.
  15. Zaghloul A, El-Shattawy H, Kassem A. Honey, a prospective antibiotic: extraction, formulation, and stability.Pharmazie 2001; 56:643?647.
  16. Khalil MI, Sulaiman SA and Boukraa L. Antioxidant Properties of Honey and Its Role in Preventing Health Disorder. The Open Nutraceuticals Journal 2010; 3: 6-16.
  17. Mart?n-Mu?oz M F, Bartolome B, Camio?na M, Bobolea I, Garcia A M C, Quirce S. Bee pollen: a dangerous food for al?lergic children. Identification of responsible aller?gens. AllergologiaImmunopathologia 2010; 38: 263-265.
  18. Fern?ndez M I. Constituents of a hexane extract of Phoenix dactylifera. Photochemistry 1983; 22 (9): 207?2088.
  19. Moritz RFA, Southwick E E. Bees a super organisms. An evolutionary reality, first ed., Springer-Verlag, Berlin; 1992.
  20. Mărghitaş L A. Produseleapicoleşiprincipalele Med. 2008;82: 384?385.
  21. ?zcan M, Ayar A. Effect of propolis extracts on butter stability. J Food Quality 2003; 26 (1): 65-73.
  22. Kamal S S, Kaur D, Singh S, Sharma A, Katual M K, Garg A K, Kumar R. An Investigative and Explanatory Review on Use of Milk as a Broad-Spectrum Drug Carrier for Improvement of Bioavailability and Patient Compliance. J Young Pharmacists. 2016; 8(2):72-75.
  23. Sun Y. Structure and biological activities of the polysaccharides from the leaves, roots and fruits of Panax ginseng CA Meyer: An overview. CarbohydrPolym 2007; 85: 490-499.
  24. Uno K, Nicholls SJ. Biomarkers of inflammation and oxidative stress in atherosclerosis. Biomark Med 2010; 4: 361-373.
  25. ,2006. Official Methods of Analysis, 18thed. Association of the Official Analytical Chemists.Washington, D.C., USA.
  26. Huang C-C, Chen Y-M, Wang D-C, Chiu C-C,Lin W-T, Huang C-Y, Hsu M-C. Cytoprotective Effect of American Ginseng in a Rat EthanolGastric Ulcer Model. Molecules. 2014; 19: P 316-326.
  27. Sabiu S, Garuba T, Sunmonuc T, Ajania E, Sulymana A, Nuraina I, Balogun A. Indomethacin-induced gastric ulceration in rats: Protectiveroles of Spondiasmombin and Ficus exasperate. Toxicology Reports 2015; 2: 261?267.
  28. Humason G L. Animal Tissue Techniques. 3rd edition. W. H. Freeman Company, San Francisco; 1972.
  29. Arab H H, Salama S A, Omar H A, Arafa E A, Maghrabi I A. Diosmin Protects against Ethanol-Induced Gastric Injury in Rats: Novel Anti-Ulcer Actions. PLoS ONE 2015; 10(3):e0122417.
  30. Schreiber S, ScheidP. Gastric mucus of the guinea pig: proton carrier and diffusion barrier, The Am J PhysiolGastrointest Liver Physiol 1997; 272(1): G63?G70.
  31. L?llmann H, Mohr K, Ziegler A, Bieger D. Color atlas of pharmacology, 2nd ed., Thieme Stuttgart, New York, pp. 166. 2000.
  32. Schlorff EC, Husain K, Somani SM. Dose- and time-dependent effects of ethanol on plasma antioxidant system in rat. Alcohol 1999;17:97?105
  33. Recknagel RO, Ghoshal AK. Quantitative estimation of peroxidative degeneration of rat liver microsomal and mitochondrial lipids after carbon tetrachloride poisoning. ExpMolPathol 1996; 5:413?26.
  34. Zakaria Z A, Balan T, Mamat SS, Mohtarrudin N, Kek T L, Salleh M Z. Mechanisms of gastroprotection of methanol extract of Melastomamalabathricum leaves. BMC Complement. Altern Med 2015; 15: 135.
  35. J?unior F E B, De Oliveira D R, Boligon A A, Athayde ML, Kamdem JP, Macedo GE, da Silva GF, de Menezes IR, Costa JG, Coutinho HD, Kerntopf MR, Posser T. Protective effects of Croton campestris A. St-Hill in different ulcer models in rodents: evidence for the involvement of nitric oxide and prostaglandins. J Ethnopharmacol 2014; 153(2): 469?477.
  36. Nordin N, Salama S M, Golbabapour S, Hajrezaie M, Hassandarvish P, Kamalidehghan B, Majid NA, Hashim NM, Omar H, Fadaienasab M, Karimian H, Taha H, Ali HM, Abdulla MA. Antiulcerogenic effect of methanolic extracts from Enicosanthellumpulchrum (King) Heusden against ethanol-induced acute gastriclesion in animal models. PLoS ONE 2014; 9(11): e111925
  37. O?Donnell VB, ChumleyPH, Hogg N, Bloodsworth A, Darley-Usmar VM, Freeman BA. Nitric oxide inhibition of lipid peroxidation: Kinetics of reaction with lipid peroxyl radicals and comparison with alpha-tocopherol. Biochemistry 1997; 36: 15216-23.
  38. Calatayud S, Barrachina D, Esplugues JV. Nitric oxide: relation to integrity, injury, and healing of the gastric mucosa. Microsc Res Tech 2001; 53:325?35.
  39. Wallace J L, McKnight W, Wilson TL, del Soldato PD, CirinoG.Reduction of shock-induced gastric damage by a nitric oxide-releasing aspirin derivative: role of neutrophils. Am J Physiol 1997; 273(6): G1246?G1251.
  40. Abdulla MA, Ali HM, Ahmed A, Noor SM, Ismai l. Evaluation of the anti-ulcer activities of Morusalba extracts in experimentally-induced gastric ulcer in rats. Biomed Res India 2009; 20: 35?39.
  41. Goswami M, Kulshreshtha M, Rao CV, Yadav S. Anti-ulcer potential of Lawsoniainermis L. Leaves against gastric ulcers in rats. J appl pharmacy sci 2011; 1: 69?72.
  42. Kobayashi T, Ohta Y, Yoshino J, Nakazawa S. Teprenone promotes the healing of acetic acid-induced chronic gastric ulcers in rats by inhibiting neutrophil infiltration and lipid peroxidation in ulcerated gastric tissues. Pharmacol Res 2001; 43: 23-30.
  43. Kang J-W, Yun N, Han H-J, Kim J-Y, Kim J-Y, S-M Lee1.Protective Effect of FlosLonicerae against Experimental Gastric Ulcers in Rats: Mechanisms of Antioxidant and Anti-Inflammatory Action. Evid Based Complement Alternat Med 2014; 24: 2014-596920.
  44. Li W, Huang H, Niu X, Fan T, Mu Q, Li H. Protective effect of tetrahydrocoptisine against ethanol induced gastric ulcer in mice. ToxicolApplPharmacol 2013; 272(1): 21?29.
  45. Hasgul R, Uysal S, Haltas H, Akyol S, Yuksel Y, Gurel A, Armutcu F. Protective effects of Ankaferd blood stopper on aspirin-induced oxidative mucosal damage in a rat model of gastric injury. ToxicolIndHealth , 2014;30(10):888?895.
  46. Gramenzi A, Caputo F, Biselli M, Kuria F, Loggi E, Andreone P, Bernardi M. Alcoholic liver disease: patho?physiological aspects and risk factors. Aliment PharmacolTher 2006; 24:1151-1161.
  47. Purohit V, Gao B, Song BJ. Molecular mechanisms of alcoholic fatty liver. Alcohol ClinExp Res 2009; 33:191-205.
  48. Baldi E, Burra P, Plebani M, Salvagnini M. Serum malo?ndialdehyde and mitochondrial aspartate aminotrans?ferase activity as markers of chronic alcohol intake and alcoholic liver disease. Ital J Gastroenterol 1993; 25:429-432.

[Enas A. El-Hussieny, EmanF.Mohamed, NeamahR.Attala and Fawzia A. A.Abd El-Rahman. (2017); GASTROPROTECTIVE EFFECT OF A NEW FORMULATED MILK TABLET ON ETHANOL-INDUCED GASTRIC MUCOSAL INJURY IN RATS. Int. J. of Adv. Res. 5 (Mar). 2374-2388] (ISSN 2320-5407). www.journalijar.com

Enas A. El-Hussieny
Zoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt

DOI:

Article DOI: 10.21474/IJAR01/3767      
DOI URL: https://dx.doi.org/10.21474/IJAR01/3767

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