Estimation of Genetic Diversity in commercial Trifolium repens reported from Pakistan using Biochemical Makers (SDS-PAGE)

Conservation of plant genetic resources has been widely exercised. The aim of the present study was to estimation the level of genetic diversity among 11 commercial Trifolium repens genotypes collected from different areas of Khyber Pakhtunkhwa, Pakistan through Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis. Based on proteomics homology; cluster analysis grouped 11 cultivars into two Lineages. Lineage-I gathered 7 cultivars, which was further divided into two clusters. Cluster-1 having 3 cultivars collected from Bannu1, Bannu3 and North Waziristan2. On the other hand cluster-2 sorted 4 cultivars collected from Bannu2, Dir Upper2 and Dir Upper3. Similarly, Linkage-ll genetically grouped 4 cultivars, which were collected from Sargoda, Mardan, Dir (L) and Dir (U)1. SDS-PAGE revealed a low level of genetic variation within commercial Trifolium cultivar collected from different agro-ecologically zones of Khyber Pakhtunkhwa, Pakistan.