Vol. 5 (04) pp. 612-622 DOI: 10.21474/IJAR01/3855

RNA SEQUENCING AND EUKARYOTIC ORGANISMS, WITH SPECIAL REFERENCE TO HAEMONCHUS CONTORTUS: A MINIREVIEW.

, , , , and
  • National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
  • Faculty of Science, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt.
  • College of Science and Arts in Unaizah, Qassim University, Unaizah, Saudi Arabia.
  • College of Applied Health Sciences in Ar Rass, Qassim University, Ar Rass 51921, Saudi Arabia.
  • Asmarya University for Islamic Sciences, Zliten, Misrata, Libya.
  • College of Animal Sciences and Technology, China Agricultural University (CAU), Beijing 100193, China.
  • College of information science and technology, Beijing normal university, Beijing, china.
  • Department of Computer Science and Information Technology, University of Management Sciences and Information Technology, Kotli Azad Kashmir, 11100, Pakistan
  • State Key Laboratory of Agricultural Microbiology, Key Laboratory of Development of Veterinary Products, Ministry of Agriculture, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei,China.
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Abstract

Next-generation sequencing (NGS) technologies are now being employed to disclose the continually changing in the transcriptional profile of a biological sample in an approach called RNA sequencing (RNA-seq). Here, we review how gene expression technology is shifting from conventional to contemporary sequencing approaches. We also provide a snap shot about the application of RNA-seq technology for discovery and comparison of gene expression patterns in different organisms with a particular emphasis on the impact of Haemonchus contortus on ovine hosts. Furthermore, we presented steps in conducting RNA-seq experiment and how to validate the RNA-seq data. A future study investigating of Haemonchus and its ovine host using RNA-seq technology would be very interesting.

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How to Cite This Article

Saeed El-Ashram, Ibrahim Al Nasr, Fathi Abouhajer, Rashid mehmood, Min Hu and Xun Suo. (2017); RNA SEQUENCING AND EUKARYOTIC ORGANISMS, WITH SPECIAL REFERENCE TO HAEMONCHUS CONTORTUS: A MINIREVIEW., Int. J. of Adv. Res., 5 (04), 612-622, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/3855

Corresponding Author

saeed_elashram

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