Studies on the identification of Bifidobacteria isolated from Human Breast milk of Indian women.
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Breast milk is an important nutrient source for neonates and many studies showed that this has beneficial effects on human health. One reason is that this micro flora of human breast milk contains the beneficial Bifidobacteria. Based on this information we proposed to isolate and identify various Bifido?s from human breast milk. In this present study a total of about 59 Breast milk samples were collected from lactating women during the first week of delivery. The milk samples of about 3ml taken into a sterile tube and subjected for screening of microflora by using standard methods. We have observed mixed flora in these samples therefore we have used a selective media i.e. Bifidobacterium agar supplemented with mupirocin. After incubation the colonies were picked up for Gram?s staining, F6PPK tests. Further the DNA was extracted from the identified Bifido?s and subjected to PCR using specific primers. Further the genome sequence analysis was carried out to know the variations which were then compared with already existing Bifidobacterium species in databases. The Bifido?s identified as ?Y? & ?V? shaped in morphology were Gram positive and the F6PPK test is positive reaction for the Genus Bifidobacterium which was further confirmed by PCR. The DNA sequencing results revealed that isolates from 10 samples out of 59 had Bifido?s. In these (6) Bifido?s had 99% similarity & 4 were found to have 98% similarity with the existing Bifidobacterium animalis sub sps lactis.Breastmilk is a potential source for Bifidobacteria particularly Bifidobacterium animalis sub sps lactis in Indian women.
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[M SHIVAPRAKASH, G MADHAVI, K B CHATHYUSHYA, G SUMALATA, S NISHANTH KUMAR & R HEMALATHA. (2017); Studies on the identification of Bifidobacteria isolated from Human Breast milk of Indian women. Int. J. of Adv. Res. 5 (May). 849-856] (ISSN 2320-5407). www.journalijar.com
Department of Microbiology & Immunology, National Institute of Nutrition (ICMR) Jamai Osmania, P.O.Tarnaka, Hyderabad-500 007, Telangana, India.
