FOLIAR REGENERATION IN CENTELLA ASIATICA (L.) URBAN (APIACEAE): AN IMPORTANT THREATENED MEDICINAL HERB.
- Department of Botany, KAHM Unity Women?s College, Manjeri, Malappuram, Kerala, India ? 676122.
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Abstract
A tissue culture protocol for mass propagation of Centella asiatica from leaves was worked out. Better callus induction was observed from the young pale green leaves on MS medium supplemented with 1mg/l 2,4-D, 0.5mg/l KIN and 4mg/l TRY. Calli were non-embryogenic, granular and friable. No callus induction was observed on MS medium without hormones. Maximum shoot regeneration from callus was observed on MS medium supplemented with 1mg/l KIN and 0.1mg/l BAP while the supply of NAA and BAP reduced the regeneration of multiple shoots. Present study prospects the in vitro conservation of C. asiatica and provides a novel source for the extraction of medicinally important secondary metabolites.
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How to Cite This Article
Deepa Palengara. (2017); FOLIAR REGENERATION IN CENTELLA ASIATICA (L.) URBAN (APIACEAE): AN IMPORTANT THREATENED MEDICINAL HERB., Int. J. of Adv. Res., 5 (05), 970-974, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/4206
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