Adverse Effect of Diazepam on Immunization of Mice Fed Diet Supplement with Chitosan

The objective of the study was to know the impact of diazepam on the immune response and the role if immune stimulation with or without Chitosan on the toxic effect of diazepam. For this purpose 60 mice both sexes, and aged (7-8) weeks were randomly divided into 4 groups and treated as follows. The first group (n=15) as immunized by the killed vaccine of Pasteurella multocida injected (0.1ml) I/P at a dose of 3 × 108 cfu/ml two doses at two weeks interval and administrated daily with diazepam for a period of eight weeks. The second group (n=15) was administrated orally with diazepam (0.6 mg/kg B. W.) for a period of eight weeks by stomach tubes and vaccinated as in the first group and fed a diet containing Chitosan (1 gm/kg). The third group (n=15) was vaccinated with Pasteurella vaccine and considered as a positive control. The forth group (n=15) was fed diets containing Chitosan and immunized with P. multocida vaccine two doses at two weeks intervals. At 28 days post immunization cell mediate immunity response determined. The mean thickness of skin at 24 and 48 hrs were 1st group (0.25±0.01) (0.12±0.01); 2nd group (0.73±0.03) (0.55±0.04); 3rd group (0.51±0.02) (0.32±0.03) and 4th group (1.10±0.09) (0.75±0.04) respectively. The humeral immune response was measured at 30 and 60 days after immunization results were shown in the 1st group (115.2±37.32) (256.0±70.11); 2nd group (179.2±31.35) (716.8±125.42); 3rd group (307.20±86.82) (665.6±153.61) and 4th group (819.2±125.42) (1638.4±250.84) respectively. Conclusion: The diazepam has side effects on immune response, while Chitosan stimulating the immune response which help in preventing the adverse effects of diazepam induce in mice, also the vaccine neutralized the adverse effects of drug with or without of Chitosan in this study.