MOLECULAR AND BACTERIOLOGICAL STUDY FOR SALMONELLA TYPHI ISOLATED FROM TYPHOID PATIENT IN SAMAWA CITY.

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Salmonella typhi is the etiologic agent of typhoid fever causer estimated 16.6 million cases of morbidity and 700.000 death world wide each years the aim of the study was to study Salmonella typhi in typhoid patient by molecular and bacteriological assay .A total of 100 blood samples aged between (1-60) years ,were involved in this study .blood samples were divided into 2 samples one sample was cultured directly on brain heart infusion broth and other sample was used for serological assay (Widal test) .After that sub cultured of isolates on MacConkey agar and XLD agar and S.S agar to find the Salmonella typhi then identified by biochemical ,antibiotic sensitive test and molecular assay by flic gene . Results showed that there was 7 Salmonella typhi isolates from blood culture and flic gene success in amplification of 363pb fragment from Salmonella typhi isolates culture that were studied ,while 36 blood samples were positive for widal test , also our isolates showed sensitivity 100% for ceftriaxone , cefepime ,cefazolin ,chloramphenicol and impenem , while all isolates were resistant 100% for tetracycline and erythromycin.

  1. Qamar, F. N., Azmatullah, A., Kazi, A. M., Khan, E. and Zaidi, A. K. M. (2014). A three-year review of antimicrobial resistance of Salmonella enterica serovars Typhi and Para typhi A in Pakistan. The Journal of Infection in Developing Countries,8: 981-986.
  2. T. 1992. health education workshops pay for public and practitioners. Nurse Pract 17:11, 15.
  3. Brooks, GF, Carroll, KC, Butel, JS, Morse, SA (2007) Medical Microbiology . 24th Edition. McGraw-Hill Companies, Inc. San Francisco. Pp. 145-150.
  4. Parkhill, J., Dougan, G., James, K. D., Thomson, N. R., Pickard, D., Wain, J. and Barrell, B. G. (2001). Complete genome sequence of a multiple drug resistant Salmonella enterica serovar typhi CT18. Nature,413: 848-852.
  5. Deng, W., Liou, S. R., Plunkett III, G., Mayhew, G. F., Rose, D. J., Burland, V. and Blattner, F. R. (2003). Comparative genomics of Salmonella enterica serovar typhi strains Ty2 and CT18. Journal of Bacteriology,185: 2330-2337.
  6. Zaki, S. A., and Karande, S. (2011).Multidrug-resistant typhoid fever: a review. The Journal of Infection in Developing Countries,5: 324-337.
  7. Sehra, D., Sehra, S., Ralia, P. and Sehra, S. T. (2013). An altered drug resistance pattern in Salmonella typhi. American Journal of Infectious Diseases and Microbiology, 1: 84-85.
  8. Ong, S. Y., Pratap, C. B., Wan, X., Hou, S., Rahman, A. Y. A., Saito, J. A. and Alam, M. (2013). The genomic blueprint of Salmonella enteric subspecies enterica serovar Typhi Pstx- 12. Standards in genomicsciences,7: 483-496.
  9. Andualem G, Abebe T, Kebede N , Gebre-Selassie S, Mihret A,Alemayehu H.A comparative study of widal test with blood culture in the diagnosis of typhoid fever in febrile patient .BMC Research Notes 2014;7(653).
  10. Song, J.H.;Cho, H.;Park, M.Y.; Na,D. S.;Moon,H.B.; Pai,C.H. (1993). Detection of Salmonella typhi in the blood of Patients with typhoid fever by polymerase chain reaction. J. Clin. Microbiol.31,1439-1443.
  11. Frana TS, Carlson SA, Griffith RW (2001) Relative distribution and conservation of genes encoding aminoglycoside-modifying enzymes in Salmonella enterica serotype Typhimurium phage type DT104. Appl Environ Microbiol 67: 445-448.
  12. AL-Tememy, T.M.K.(2001).Isolation ,purification of two specific antigens from the local isolate Salmonella typhi and using them in the enzyme-linked immunosorbent assay as serological diagnosis for typhoid fever .M.S.c.(thesis). College of Science/University of Basrah.
  13. Berkley, J. A., Lowe, B. S., Mwangi, I., Williams, T., Bauni, E., Mwarumba, S., Ngetsa, C., Slack, M. P. E., Njenga Sally, Hart, C. A., Maitland, K., English, M., Kevin, M. and Scott, J. A. G., (2005). Bacteremia in children admitted to a rural hospital in Kenya .The New England Journal of Medicine, 352(1):39-47.
  14. J. and Hosoglu S.(2008). The Laboratory diagnosis of enteric fever. J. Infect Developing Countries. 2(6):241-425.
  15. Subhei, H.T.(2010). Microbiological and molecular identification of Salmonella enteric serovar Typhi: in Clinically suspected Typhoid Patients in Garmiyan district. MSC. Thesis, College of Science, University of Sulaimani, Iraq.
  16. Song, J.H.;Cho, H.;Park, M.Y.; Na,D. S.;Moon,H.B.; Pai,C.H. (1993). Detection of Salmonella typhi in the blood of Patients with typhoid fever by polymerase chain reaction. J. Clin. Microbiol.31,1439-1443.
  17. Arora, D. R. and Arora, B. (2011). A text book of Microbiology. 3rd Edition, CBS publishers PV Ltd, New Delhi, India. Pp 352, 368-373, 376, 382, 385, 410-412.
  18. Raveendran, R., Datta, S. and Chand, W. (2010). Drug Resistance in Salmonella enterica serotype typhi and para typhi IJMSA,23: 21-24.

[Ali Anook and Alaa Farooq. (2017); MOLECULAR AND BACTERIOLOGICAL STUDY FOR SALMONELLA TYPHI ISOLATED FROM TYPHOID PATIENT IN SAMAWA CITY. Int. J. of Adv. Res. 5 (Jun). 692-699] (ISSN 2320-5407). www.journalijar.com

Alaa Farooq Rasool

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Article DOI: 10.21474/IJAR01/4467      
DOI URL: https://dx.doi.org/10.21474/IJAR01/4467

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