ISOLATION AND CHARACTERIZATION OF CARBENDAZIM-DEGRADING BACTERIA IN RICE PADDY SOIL IN CAN THO, VIETNAM.

  • Department of Biology, College of Natural Sciences, Can Tho University, 3/2 street, Xuan Khanh ward, Ninh Kieu district, Can Tho city, Vietnam.
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Carbendazim is one of the most commonly used fungicides in Vietnam in recent decades. The intensive application of this pesticide has led to the increase of its presence in soil, surface water, and ground water. This study reports the isolation and characterization of carbendazim-degrading bacterial isolates obtained from pesticide-treated rice paddy soil in Can Tho, Vietnam. Three bacterial isolates capable of utilizing carbendazim as sole carbon source and performing chemotaxis towards carbendazim were isolated. All of them were able to degrade 90% of carbendazim at concentration of 200 mg L-1 after 6 days of incubation. These isolates were identified as Pseudomonas sp. CT-VT7, Acinetobacter sp. CT-VT9, and Stenotrophomonas sp. CT-VT13 based on their 16S-rRNA gene sequences. The results indicate that these strains are potential candidates for carbendazim biodegradation and could be used for applied study on carbendazim removal in the environment.

  1. Alvarado-Guti?rrez, M. L., Ruiz-Ordaz, N., Gal?ndez-Mayer, J., Santoyo-Tepole, F., Curiel-Quesada, E., Garc?a-Mena, J., &Ahuatzi-Chac?n, D. (2017). Kinetics of carbendazim degradation in a horizontal tubular biofilm reactor. Bioprocess and Biosystems Engineering, 40(4), 519-528.
  2. Arya, R., & Sharma, A. K. (2015). Bioremediation of carbendazim, a benzimidazole fungicide using Brevibacillus borstelensis and Streptomyces albogriseolus Current Pharmaceutical Biotechnology, 17(2), 185-189.
  3. Baude, F. J., Pease, H. L., & Holt, R.F. (1974). Fate of benomyl on field soil and turf. Journal of Agricultural and Food Chemistry, 22(3), 413-418.
  4. Berglof, T., Van Dung, T., Kylin, H., & Nilsson, I. (2002). Carbendazim sorption-desorption in Vietnamese soils. Chemosphere, 48(3), 267-273.
  5. Breugelmans, P., & Uyttebroek, M. (2004). Protocol for DNA extraction and purification. Laboratory of soil and water management, KU Leuven, Belgium.
  6. Cancela, G. D., Taboada, E. R., & S?chez-Rasero, F. (1992). Carbendazim adsorption on montmorillonite, peat and soils. Journal of Soil Science, 43, 99-111.
  7. Fang, H., Wang, Y., Gao, C., Yan, H., Dong, B., & Yu, Y. (2010). Isolation and characterization of Pseudomonas CBW capable of degrading carbendazim. Biodegradation, 21(6), 939-946.
  8. Fleeker, J. R., Lacy, H. M., Schultz, I. R. & Houkom, E. C. (1974). Persistence and metabolism of thiophanate-methyl in soil. Journal of Agricultural and Food Chemistry, 22, 592-595.
  9. Grimm, A., & Hardwood, C. S. (1997). Chemotaxis of Pseudomonas to the polyaromatic hydrocarbon naphthalene. Applied and Environmental Microbiology, 63(10), 4111-4115.
  10. Habe, H., & Omori, T. (2005). Genetic of polycyclic aromatic hydrocarbon metabolism in diverse aerobic bacteria. Bioscience, Biotechnology, and Biochemistry, 67(2), 225-243.
  11. Helweg, A. (1977). Degradation and adsorption of carbendazim and 2-aminobenzimidazole in soil. Pesticide Science, 8, 71-78.
  12. Lamers, M., Anyusheva, M., La, N., Nguyen, V.V., & Strect, T. (2011). Pesticide pollution in surface- and groundwater by paddy rice cultivation: a case study from Nothern Vietnam. Clean ? Soil, Air, Water, 39(4), 356-361.
  13. Liu, F. Y., Hong, M. Z., Liu, D. M., Li, Y. W., Shou, P. S., Yan, H., & Shi, G. Q. (2007). Biodegradtion of methyl parathion by Acinetobacter radioresistens USTB-04. Journal of Environmental Science, 19(10), 1257-1260.
  14. Mazellier, P., Leroy, E., Laat, J.T., & Legube, B. (2003). Degradation of carbendazim by UV/H2O2 investigated by kinetic modeling. Environmental Chemistry Letters, 1, 68-72.
  15. Minh, T.B., Iwata, H., Takahashi, S., Viet, P.H., Tuyen, B.C., & Tanabe, S. (2008). Persistent organic pollutants in Vietnam: environmental contamination and human exposure. Reviews of Environmental Contamination and Toxicology, 193, 213-290.
  16. Morinaga, H., Yanase, T., Nomura, M., Okabe, T., Goto, K., Harada, N., & Nawata, H. (2004). A benzimidazole fungicide, benomyl, and its metabolite, carbendazim, induce aromatase activity in a human ovarian granulose-like tumor cell line (KGN). Endocrinology, 145(4), 1860-1869.
  17. Nakai, M., Hess, A. R., Moore, J. B., Gutroff, F. G., Strader. F. L., & Linder, E. R. (1992). Acute and long-term effects of a single dose of the fungicide carbendazim (methyl 2-benzimidazole carbamate) on the male reproductive system in the rat. Journal of Andrology, 13(6), 507-518.
  18. Nakai, M., Miller, M. G., Carnes, K., & Hess, R. A. (2002). Stage-specific effects of the fungicide carbendazim on Sertoli cell microtubules in rat testis. Tissue Cell, 34(2), 73-80.
  19. Nguyen, T. P. O. (2014). Genetic assessment of pesticide-degrading bacteria in the Mekong delta of Vietnam. PhD thesis. KU Leuven, Belgium. ISBN 978-90-8826-370-5.
  20. Pailan, S., Sengupta, K., Ganguly, U., & Saha, P. (2016). Evidence of biodegradation of chlopyrifos by a newly isolated heavy metal-tolerant bacterium Acinetobacter sp. strain MemCl4. Environmental Earth Sciences, 75(12), 1-10.
  21. Parales, R. E., Ditty, J. L., & Hardwood, C. S. (2000). Toluene-degrading bacteria are chemotactic towards the environmental pollutants benzene, toluene, and trichloroethylene. Applied and Environmental Microbiology, 66(9), 4098-4104.
  22. Rajeswary, S., Kumaran, B., Ilangovan, R., Yuvaraj, S., Sridhar, M., Venkataraman. P., Srinivasan, N., & Aruldhas, M. M. (2007). Modulation of antioxidant defense system by the environmental fungicide carbendazim in Leydig cells of rats. Reproductive Toxicology, 24(3-4), 371-380.
  23. Salunkhe, V. P., Sawant, I. S., Banerjee, K., Wadkar, P. N., Sawant, S. D., & Hingmire, S. A. (2014). Kinetics of degradation of carbendazim by B. subtilis strains: possibility of in situ detoxification. Environmental Monitoring and Assessment, 186(12), 8599-8610.
  24. Singh, P., Suri, C. R., & Cameotra, S. S. (2004). Isolation of a member of Acinetobacter species involved in atrazin degradation. Biochemical and Biophysical Research Communications, 317(3), 697-702.
  25. Singhal, L. K., Bagga, S., Kuma, R., & Chauhan, R. S. (2003). Down regulation of humoral immunity in chickens due to carbendazim. Toxicology in Vitro, 17(5-6), 687-692.
  26. Solel, Z., Sandler, D., & Dinoor, A. (1979). Mobility and persistence of carbendazim and thiabendazole applied to soil via drip irrigation. Phytopathology, 69(12), 1273-1277.
  27. Sun, J., Xu, J., Hu, H., & Hu, M. (2013). Bacterium for efficiently degrading residual pesticide carbendazim and application threof. Chinese Patent No. CN1022906041 B. China Patent and Trademark Office.
  28. Thuy, P. T., Van Geluwe, S., Nguyen, V., &Van der Bruggen, B. (2012). Current pesticide practices and environmental issues in Vietnam: management challenges for sustainable use of pesticides for tropical crops in (South-East) Asia to avoid environmental pollution. Journal of Material Cycles and Waste Management, 14(4), 379-387.
  29. Toan, P. V., Sebesvari, Z., Bl?sing, M., Rosendahl, I., Renaud, F. G. (2013). Pesticide management and their residues in sediments and surface and drinking water in the Mekong Delta, Vietnam. Science of the Total Environment, 452-453, 28-39.
  30. Wang, Z., Wang, Y., Gong, F., Zhang, J., Hong, Q., &Li, S. (2010). Biodegradation of carbendazim by a novel actinobacterium Rhodococcus jialingiae djl-6-2. Chemosphere, 81(5), 639-644.
  31. Wilmotte, A., Van der Auwera, G., &De Wachter, R. (1993). Structure of the 16 S ribosomal RNA of the thermophilic cyanobacterium Chlorogloeopsis HTF ('Mastigocladus laminosus HTF') strain PCC7518, and phylogenetic analysis. FEBS Letters, 317(1-2), 96-100.
  32. Xie, S., Liu, J., Li, L., & Qiao, C. (2009). Biodegradation of malathion by Acinetobacter johnsonii MA19 and optimization of cometabolism substrates. Journal of Environmental Sciences (China), 21(1), 76-82.
  33. Xu,. J. L., Gu, X. Y., Shen, B., Wang, Z. C., Wang, K., & Li, S. P. (2006). Isolation and characterization of a carbendazim-degrading Rhodococcus sp. djl-6. Current Microbiology, 53(2006), 72-76.
  34. Yarden, O., Salomon, R., Katan, J. & Aharonson, N. (1990). Involvement of fungi and bacteria in enhanced and nonenhanced biodegradation of carbendazim and other benzimidazole compounds in soil. Canadian Journal of Microbiology, 36, 15-23.
  35. Yu, G., Liu, Y., Xie L., & Wang, X. (2009). Involvement of Sertoli cells in spermatogenic failure induced by carbendazim. Environmental Toxicology and Pharmacology, 27(2), 287-292.
  36. Zhang, G. S., Jia, X. M., Cheng, T. F., Ma, X. H., & Zhao, Y. H. (2005). Isolation and characterization of a new carbendazim-degrading Ralstonia sp. strain. World Journal of Microbiology and Biotechnology, 21(3), 265-269.
  37. Zhang, X., Huang, Y., Harvey, P. R., Li, H., Ren, Y., Li, J., Wang, J., & Yang, H. (2013). Isolation and characterization of carbendazim-degrading Rhodococcus erythropolis djl-11. PLoS ONE, 8(10), e74810.

[Hong Phuong Le, Thanh Khang Le and Thi Phi Oanh Nguyen. (2017); ISOLATION AND CHARACTERIZATION OF CARBENDAZIM-DEGRADING BACTERIA IN RICE PADDY SOIL IN CAN THO, VIETNAM. Int. J. of Adv. Res. 5 (Jun). 863-870] (ISSN 2320-5407). www.journalijar.com

Thi Phi Oanh NGUYEN
Department of Biology, College of Natural Sciences, Can Tho University, Viet Nam

DOI:

Article DOI: 10.21474/IJAR01/4486      
DOI URL: https://dx.doi.org/10.21474/IJAR01/4486

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