EFFECT OF GLUCOSE ON SPERM MOTILITY IN DANIO RERIO AS A MODEL OF DIABETES: A PRELIMINARY STUDY.

  • Department of Life Science and Biochemistry, St. Xavier?s College - Autonomous, Mumbai, India.
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Diabetes mellitus impairs reproductive function by causing low sperm count, seminal volume, and sperm motility. Zebrafish (Danio rerio) is a potential model organism for diabetes, and the normal glucose levels in zebrafish and human semen are known. However, the glucose levels in semen that cause adverse effects in diabetes are unclear. Therefore, this study aimed to evaluate whether there is a direct effect of glucose on sperm motility and determine the glucose concentration that shows a significant effect on sperm motility and morphology by mimicking diabetes-like conditions in zebrafish in vitro. A two-part experiment was conducted: (a) Quantitative study, wherein sperm motility was examined at different glucose concentrations. (b) Qualitative study, wherein sperm morphology was visualized using Eosin Y staining. Data were analyzed using analysis of variance and post-hoc Dunnett?s test. The results showed that sperm motility significantly diminished with 5-h incubation in 60 mM glucose. Concentrations of 1?5 mM glucose did not have any effect on sperm motility at 2, 3, or 5 h. However, several morphological abnormalities were observed across glucose concentrations and incubations. In conclusion, glucose at a concentration of 60 mM with 5-h incubation shows a change in zebrafish sperm motility. These findings may contribute to the development of zebrafish as a model of diabetes.

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[Candice Judith D?costa and Manasi Kanuga. (2017); EFFECT OF GLUCOSE ON SPERM MOTILITY IN DANIO RERIO AS A MODEL OF DIABETES: A PRELIMINARY STUDY. Int. J. of Adv. Res. 5 (Jun). 823-832] (ISSN 2320-5407). www.journalijar.com

Manasi Kanuga
Department of Life Science and Biochemistry, St. Xavier’s College - Autonomous, Mumbai, India

DOI:

Article DOI: 10.21474/IJAR01/4480      
DOI URL: https://dx.doi.org/10.21474/IJAR01/4480

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