CLINICOPATHOLOGICAL SIGNIFICANCES OF STEM CELL MARKERS SOX-2& PIWIL1 EXPRESSIONS IN ENDOMETROID CARCINOMA OF THE UTERUS.

  • Department of Pathology, Faculty of Medicine, Beni-Suef University, Egypt.
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Background: Endometroid carcino?ma (EMC) is the commonest subtype of endometrial cancer. Recent researchers had proved the role of cancer stem cells (CSCs) in its carcinogenesis. There are many stem cell-related transcription factors, such as Sex-determining region y (SRY)-Box2 (SOX2) which is a SOX family member that had many roles in controlling stem cell proper?ties and differentiation. But, the accurate clinicopathological role of SOX-2 in endometroid carcinoma is still not sufficiently clarified. The epithelial-mesenchymal transition (EMT) is the process in which the epithelial cell loses its polarity and cell to cell adhesions, and having motile criteria. Recently, a link was found between EMT and acquiring stem cell-like properties of tumor cells. The Piwil1 gene is a Piwi gene family member that was found to be required for self-renewal and division of stem cell. Proving Piwil1 roles in EMT and acquiring stem cell-like properties in EMC need more clarification aiming at understanding endometrial carcinogenesis. The aim of the work: to evaluate stem cell markers SOX-2 and Piwil1 co-expression in endometroid carcinoma (EMC) and to assess the correla?tion between their expressions and clinocopathological features of such type of malignancy. Patients And Methods: we assesses SOX-2 and Piwil1 co-expression by using immunohistochemistry in sections from fifty blocks of paraffin of EMC then analyzed correlations between the levels of SOX-2 and Piwil1 co-expression and the clinicopathological parameters of patients. Results: High expression of SOX-2 was detected in 28 out of 50 (56%) and high expression f Piwil1 was detected in 32 out of 50 (64%) cases of EMC. High expression of SOX-2 was significantly positively correlated with higher grade& advanced stage of the tumor, presence of L.N metastases& distant metastases (p<0.001), presence of myometrial (p=0.002), lymphovascular (p=0.006) & parametrial (p=0.008), serosal (p=0.04), cervical stromal (p=0.003) & adnexal invasion (p=0.002). High expression f Piwil1 was significantly positively correlated with the presence of distant metastases (p=0.009), higher grade (p=0.002) & advanced stage of the tumor, presence of L.N metastases (p<0.001), presence of myometrial (p=0.008), lymphovascular (p=0.006), parametrial, serosal (p=0.005), cervical stromal (p=0.003) and adnexal invasion (p=0.002). Expression of SOX2 were significantly positively correlated with Piwil1 (p<0.001). Conclusion: SOX-2& Piwil 1 co-expression correlates with poor clinicopathological parameters of endometroid carcinoma.

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[Dina A. Khairy and Abla S. Mahmoud. (2017); CLINICOPATHOLOGICAL SIGNIFICANCES OF STEM CELL MARKERS SOX-2& PIWIL1 EXPRESSIONS IN ENDOMETROID CARCINOMA OF THE UTERUS. Int. J. of Adv. Res. 5 (Jun). 1025-1037] (ISSN 2320-5407). www.journalijar.com

Dina A. Khairy
lecturer of Pathology, Faculty of Medicine, Beni-Suef University

DOI:

Article DOI: 10.21474/IJAR01/4505      
DOI URL: https://dx.doi.org/10.21474/IJAR01/4505

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