COMPARATIVE STUDY OF LED FLUORESCENT MICROSCOPY WITH THE CONVENTIONAL ZN BASED MICROSCOPY FOR DIAGNOSIS OF MICROBIOLOGICALLY CONFIRMED TB CASES.

  • Department of Biotechnology, College of Commerce, Arts & Science (Magadh University, Bodh Gaya), Patna, Bihar, India.
  • Intermediate Reference Laboratory, TBDC building, Patna, Bihar, India.
  • Department of Virology, Rajendra Memorial Research Institute of Medical Sciences (ICMR), Agam kuan, Patna, India.
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Conventional light microscopy of Ziehl Neelsen (ZN) stained smears prepared directly from sputum specimens is the most widely available test for diagnosis of microbiologically confirmed tuberculosis (TB) in India. The ZN based microscopy needs concentration of 5000 ? 10000 acid fast bacilli per ml for its detection and hence reported to detect only 60 ? 70 % TB cases. On the other hand, LED Fluorescent Microscopy (LED FM) with auramine based staining claims to be a faster and more sensitive technique than the conventional microscopy. In the present work, we have evaluated both the techniques taking culture as the reference standard. We have taken sputum samples of 300 presumptive MDR TB patients and tested by all three techniques. 120 samples were positive by ZN microscopy. Along with these, additional 21 samples were reported positive by LED FM. Out of 300 samples inoculated on culture, 169 samples turned positive. All the culture negative samples were smear negative by both the microscopy techniques. So, we observed that by using LED FM, we can enhance the positivity rate up to 17.5 %. We concluded that the LED FM is comparatively more sensitive technique than the conventional light microscopy. Our findings provide support to the World Health Organization (WHO) policy recommendation that conventional light microscopy to be replaced by LED fluorescent microscopy using auramine staining in all settings for clinical sample testing.

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[Pratik Kumar, Prashant Goswami, Rashmi Kumari, Maneesh Kumar and Subham Kumar. (2017); COMPARATIVE STUDY OF LED FLUORESCENT MICROSCOPY WITH THE CONVENTIONAL ZN BASED MICROSCOPY FOR DIAGNOSIS OF MICROBIOLOGICALLY CONFIRMED TB CASES. Int. J. of Adv. Res. 5 (Jun). 1083-1086] (ISSN 2320-5407). www.journalijar.com

Pratik Kumar
Intermediate Reference Laboratory, Agamkuan, Patna, Bihar, India

DOI:

Article DOI: 10.21474/IJAR01/4511      
DOI URL: https://dx.doi.org/10.21474/IJAR01/4511

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