The Protective Effect of Green Apple Juice on physiological and Biochemical Parameters in Blood of Male Albino Rats Exposed to X- Ray

The present study aimed to investigate the protective effect of green apple juice against the harmful impact resulting from exposure to X- ray in adult male albino rats Rattus norvegicus aged (5-7) months, their weight (250- 350)g. 40 male rats were used, they were divided randomly into four groups, 10 rats for each group, as follow: The first group was given standard forage and normal tap water and considered as a control, the second group was exposed to X- ray at about 76 Kv. (reduplicate) for a period of 3 days each 5 seconds, Third and fourth group were treated with green apple juice 2 and 4 ml/Kg. B.W. respectively by gavage tube, and after one hour of treatment, the rats were exposed to X- ray at about 76 Kv. (redupilcate) for a period of 3 days each 5 seconds, the last three groups were given a standard forage and tap water ad libitum. The results showed that X- ray exposure caused a significant decrease at (P? 0.05) in the haeamoglobin concentration, packed cell volume, total red blood cells count, the concentration of each of total protein, albumin, globulin and uric acid, but a significant increase in total white blood cells count, platelets count, the concentration of urea, creatinine and peroxy nitrate radical in blood of male reats exposed compared with control group (was not exposed). The results also showed a significant decrease in glutathione (GSH) concentration which has a role as antioxidant, with a significant increase in malondialdehyde (MDA) concentration in blood serum of exposed rats as compared with control group, the a above changes indicate the ability of X- ray to induce oxidative stress in male rats. The results also showed that treating rats with green apple juice 2 and 4 ml/ Kg. B.W. and then exposed them to X- ray causes a significant increase in some of the decreased hematological and biochemical parameters and vice versa, the results also showed that there was a significant increase in GSH concentration a compained with a significant decrease in MDA concentration as compared with the group exposed to X- ray only. From this study we concluded that the green apple juice has a role protecting against reactive oxygen species that are produced as a result of exposure to radiation through body's cells, which happened as a result of increasing the production of antioxidants and inhibit free radicals in the blood. So we concluded that the green apple juice had the ability to protect against X-ray side effects.