Vol. 5 (08) pp. 1709-1722 DOI: 10.21474/IJAR01/5235

SET FOR TRIPANOSOMOZ'S (SU-AURU) DIAGNOSTICS.

  • «Antigen» scientific and production enterprise, Kazakhstan, Almaty region.
  • Kazakh national agricultural university, Kazakhstan, Almaty.
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Abstract

The technology of preparation of a set for diagnostics of a trypanosomosis (su auru) which consists of the following stages is given in article: allocation of a field strain of the Trypanosoma evansi activator; maintenance of a strain of Trypanosoma evansi on experimental animals: white mice, rabbits, guinea pigs, dogs, donkeys, horses; strengthening of virulence of a strain of Trypanosoma evansi repeated passages of the activator through an organism of susceptible animals, especially dogs and donkeys, and also by introduction infected animal immunodepressants; receiving from animals infestation blood trypanosomes their draining of blood a la carte is also total; obtaining tripanosomes weight by centrifugation of blood and washing of trypanosomes weight; desintegrating and resuspension of trypanosomes weight; office of a specific trypanosomosis anti-gene; standardization of an anti-gene on serological activity; stabilization of an antigene liophilization; receiving hyper immunized (positive) serums; receiving negative serums; test for specificity and sensitivity by statement various the serological of tests: complement binding reaction (CBR), complement long binding reaction (CLBR) and the enzyme-linked immunosorbent assay (ELISA); formation of components of a set; labeling and packing.

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How to Cite This Article

Shabdarbayeva G., Akhmetsadykov N., Balgimbaeva A., Ibazhanova A., Khussainov D., Amirgaliyeva S., Turganbayeva G. and Kidirov T. (2017); SET FOR TRIPANOSOMOZ'S (SU-AURU) DIAGNOSTICS., Int. J. of Adv. Res., 5 (08), 1709-1722, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/5235

Corresponding Author

ShabdarbayevaG.
scientific and production enterprise, Kazakhstan, Almaty region.