LEAD NITRATE INDUCED DEVELOPMENTAL DEFECTS IN DROSOPHILA MELANOGASTER ACCOMPANIED WITH LARVAL CHROMOSOMAL REARRANGEMENTS.
- Department of Molecular Biology, Institute of Genetic Engineering 30, Thakurhat Road,Kolkata-700128, India.
- Department of Genetics, Institute of Genetic Engineering 30, Thakurhat Road , Kolkata-700128, India.
- Abstract
- Keywords
- References
- Cite This Article as
- Corresponding Author
Lead induced toxicity is common in human due to repeated occupational exposure of this heavy metal as well as its incorporation into the body through contaminated food and environmental pollutions. With a view to assess toxic potential of this heavy metal on development, dose dependent exposures of lead nitrate were given to a model organism, Drosophila melanogaster during its development. It was observed that at low doses lead nitrate did not affect the development of this fly. However, beyond at certain concentration, it exerted negative influences on its metamorphosis as well as in the number of pupa and emerging adults accompanied with various chromosomal abnormalities in third instar larval polytene chromosomes. From our experimental results it can be suggested that lead nitrate induced chromosomal abnormalities in larvae may cause dysfunction of developmental genes that give rise to developmental delay and reduce the the population of emerging individuals. As Drosophila melanogaster exhibit subtantial similarities with human genome, negative developmental outcome owing to a lead salt exposure in this fly may reflect analogous situation during human pregnancy.
- Abadin, H., Ashizawa, A., Stevens, Y., Llados, F., Diamond, G., Sage, G., Citra, M., Quinones. A., Bosch, S.J. and Swarts SG. (2007): Health effects. In: Toxicological Profile for Lead. Agency for Toxic Substances and Disease Registry (US). Atlanta (GA).
- Abdullahi, M. S. (2013 ): Toxic effects of lead in humans: an overview.l Adv. Res. J. Env. Sci. Toxicol,2(6) : 157-162
- Agrawal, P., Mittal, A., Kumar, M. and? Tripath, S. K . (2009): A comprehensive review of environmental exposure of Lead and its toxicity. Ind. Acad. Forensic Med, 31(3): 299-303.
- Amadi,C.N., Igweze, Z.N. and Orisakwe, O.E.(2017). Heavy metals in miscarriages and stillbirths in developing nations. Middle East FertilSoc J, 22(2): 91-100.
- Assi, M.A., Hezmee, M.N.M., HaronW., Sabri, M.Y.M. and Rajion , M.A.(2016).The detrimental effects of lead on human and animal health. Vet World, 9(6): 660?671.
- Bennett MD, Smith JB, Simpson S, Wells B. (1979). Intranuclearfibrillar material in cereal pollen mother cells. Chromosoma, 71:289?332.
- Bokor, K. andPecsenye, K. (1997) Strains of Drosophila melanogaster differ in alcohol tolerance. Hereditas,126 (2): 103-113.
- Chaudhry, A., Anand,P.K.,Geeta.,Singh.S, and Lovleen. ( 2006). Ectopic pairing of the intercalary heterochromatin in the organophosphate pesticide treated mosquito chromosomes (Culcidae: Diptera). Cytologia, 71(4): 431?437.
- Fung, J.C., Marshall, W.F.,Dernburg, A., Agard, A.D. and John W. Sedat. (1998) . Homologous chromosome pairing in Drosophila melanogaster proceeds through multiple independent initiations. J. Cell Biol., 141(1): 5?20
- Goldsborough, A.S. and Kornberg, T.B. (1996). Reduction of transcription by homologue asynapsis in Drosophila imaginal discs.Nature, 381(6585): 807-810.
- Haq, R., M. Khan, M.F. and Haq, E.(2011).Adverse? Effect of lead acetate on Drsophila melanogaster. Basic & Appl. Sci, 7(2): 15-163.
- Hirsch HV, Mercer J, Sambaziotis H, Huber M,?Stark DT,?Torno-Morley T, Hollocher K,?Ghiradella H,?Ruden DM. (2003). Behavioral effects of chronic exposure to low levels of lead in Drosophilamelanogaster. Neurotoxicology, 24: 435?442.
- Holliday, R. (1968). Genetic recombination in fungi. In Replication and Recombination of Genetic Material. W.J. Peacock and R.D. Brock, editors. Australian Academy of Science, Canberra. 157?174.
- Lead and lead compounds.Report on carcinogens, (2016). https://ntp.niehs.nih.gov/ntp/roc/content/profiles/lead
15.??? Lead poisoning - Symptoms and causes - Mayo Clinic.? http://www.mayoclinic.org/diseases-conditions/lead-poisoning/symptoms-causes/dxc-20275054
- Lu, L., Cheng, H., ?Liu, X., Xie, J., ?Li, Q. and Tan Zhou, T. (2015): ?Assessment of regional human health risks from lead contamination in Yunnan province, southwestern China. Plos one, 10(4): e0119562.? Published online 2015 Apr 20. doi:? 1371/journal.pone.0119562.
- Nanda Kumar Sasi, Michael Weinreich(2016): DNA replication Checkpoint Signalling. In: Daniel L. Kaplan (Ed) The Initiation of DNA Replication in Eukaryotes. Springer 2016Switzerland.
- Pandey, U.B. and Nichols, C.D. (2011). Human disease models in Drosophila melanogaster and the role of the fly in therapeutic drug discovery. Rev, 63(2): 411-436
- Rand MD, Dao JC, Clason TA. ( 2009). Methylmercury disruption of embryonic neural development inDrosophila. Neurotoxicol30: 794?802.
- Rand MD, Montgomery SL, Prince L, Vorojeikina D. (2014). Developmental toxicity assays using the Drosophila CurrProtocToxicol59: 1.12.1?1.12.20.
- Safaee, S.,MasoudFereidoni, A., Mahadavi-Shahri,N., Haddad, F and Mirshamsi, O.(2014). Effects of lead on the development of Drosophila melanogaster. Biol, 116(3): 259?265.
- Sahai-Hernandez P, Castanieto A, Nystul TG. (2012). Drosophilamodels of epithelial stem cells and their niches. Wiley Interdiscip Rev DevBiol, 1: 447-457.
- Tong, S., Yasmin E. von Schirnding, Y.E.V. and Prapamontol, T. (2000 ): Environmental lead exposure: a public health problem of global dimensions. World Health Organ,78 (9): 1068-1077.
- Uysal, H. (1997). Induction of chromosomal aberrations in polytene chromosomes of Drosophila melanogaster by lead acetate. Cytologia, 62(3): 213-217.
- Wani,A.L., Ara, A. and? UsmanI, J.A. (2015): Lead toxicity: a review. Toxicol., 8(2): 55?64
- Zlotorynski, E., Rahat, A.,Skaug, J., Ben-Porat, N.,Ozeri, E.,Hershberg,R., Levi, A., SScherer, S.W., Margalit, H. and BatshevaKerem. (2003).Molecular Basis for Expression of Common and Rare Fragile Sites. Mol Cell Biol, 23(20): 7143?7151.
[Ashim Kumar Basak, Tridip Chatterjee, Arijit Sarbabidyaa and Amit Chakravarty. (2017); LEAD NITRATE INDUCED DEVELOPMENTAL DEFECTS IN DROSOPHILA MELANOGASTER ACCOMPANIED WITH LARVAL CHROMOSOMAL REARRANGEMENTS. Int. J. of Adv. Res. 5 (Aug). 467-475] (ISSN 2320-5407). www.journalijar.com
Department of Molecular Biology Institute of Genetic Engineering. 30, Thakurhat Road, Kolkata-700128, India