ESTIMATION OF EXTRACELLULAR LIGNINOLYTIC ENZYMES FROM WILD AURICULERIA POLYTRICHA, HELVELLA SP. AND MORCHELLA SP.
- Applied Sciences and Biotechnology, Shoolini University.
33 Downloads
166 Views
Abstract
Three mushroom samples have been selected for extracellular ligninolytic enzyme activities i.e commercially cultivated Auricularia polytricha, wild Helvella sp. and wild Morchella sp. To investigate the extracellular ligninolytic enzymes, cultures were incubated over a period of 20 days in nutrient rich medium (NRM) and nutrient poor medium (NPM) under static conditions. Samples showed green and brown coloration in solid medium plates; it indicates the presence of laccase and Manganese peroxidase (MnP). Production of extracellular ligninolytic enzymes were observed in all three species in different pattern. The results of the present study allow us to conclude that wild Morchella sp. and Helvella sp. are good for production of ligninolytic enzymes in comparison to commercially cultivated Auricularia polytricha.
Keywords
Article Analytics
References
- Adenipekun CO, Fasidi IO (2005) Bioremediation of oil polluted soil by Lentinus subnudus, a Nigerian white-rot fungus. Afr J Biotechnol 4(8):796?798
- Adejoye OD, Adebayo-Tayo BC, Ogunjobi AA, Olaoye OA, Fadahunsi FI (2006) Effect of carbon, nitrogen and mineral sources on growth of Pleurotus florida, a Nigeria edible mushroom. Afr J Biotechnol 5(14):1355?1359
- Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principal of protein dye binding. Analyt Biochem 72:248?254
- Camarero S, Sarkar S, Ruiz-Duenas FJ, Martı?nez MJ, Martinez AT (1999) Description of a versatile peroxidase involved in natural degradation of lignin that has both Mn peroxidase and lignin-peroxidase substrate binding sites. J Biol Chem 274: 10324?10330
- Chen DM, Bastias BA, Taylor AFS, Cairney JWG 2003. Identification of laccase-like genes in ectomycorrhizal basidiomycetes and transcriptional regulation by nitrogen in Piloderma byssinum.New. Phytol. 157:547?554
- Cohen R, Hadar Y, Yarden O (2001) Transcript and activity levels of different Pleurotus ostreatus peroxidases are differentially affected by Mn2?. Environ Microbiol 3(5):312?322
- Eggert, C., U. Temp, J. F. Dean and K. E. Eriksson (1996). A fungal metabolitemediates degradation of non-phenolic lignin structures and synthetic lignin by laccase. Let. 391: 144-148.
- Espindola LHS, Espindola FS, Freitas GR, Brandeburgo MAM (2007) Biodegradation of Red 40 dye by the Pleurotus sp. Florida.Biosci J 23(3):90?93
- Farmer VC, Henderson MEK, Russell JD (1960) Aromaticalcohol- oxidase activity in the growth medium of Polystictus versicolor. Biochem J 74:257?262
- Hatakka A (2001) Biodegradation of lignin. In: Hofrichter M, Steinbu?chel A (eds) Lignin, humic substances and coal, vol 1. Wiley, Weinheim, pp 129?180
- Han M-J, Chai H-T, Song H-G (2005) Purification and characterization of laccase from the white-rot fungus Trametes versicolour.The J Microbiol 46(6):555?560
- Heinfling A, Ruiz-Duen?as FJ, Martı?nez MJ, Bergbauer M et al (1998) A study on reducing substrates of manganese-oxidizing peroxidases from Pleurotus eryngii and Bjerkandera adusta. FEBS Lett 428:141?146
- Jonathan, S.G. (2002). Vegetative growth requirements and antimicrobial of some higher fungi in Nigeria. Ph. D Thesis, University of Ibadan. p. 268
- Kim SJ, Suzuki N, Uematsu Y, Shoda M (2001) Characterization of aryl alcohol oxidase produced by dye-decolorizing fungus, Geotrichum candidum Dec1. J Biosci Bioeng 91:166?172
- Kirk TK, Cullen D (1998) Enzymology and molecular genetics of wood degradation by White-rot fungi. In: Young RA, Akthar M (eds) Environmentally friendly technologies for the pulp and paper industry. Wiley, New York, pp 273?307
- Moore, D. and S. W. Chiu (2001). Fungal products as food. Chap. 10: Bio-Exploitation of Filamentous Fungi (S. B. Pointing & K. D. Hyde editor). Fungal Diversity Press: Hong Kong. 223-251
- Martinez AT, Speranza M, Ruiz-Duenas FJ, Ferreira P et al (2005) Biodegradation of lignocellulosic: microbial chemical and enzymatic aspects of the fungal attack of lignin. Int Microbiol 8:195?204
- Mikolasch A, Schauer F (2009) Fungal laccases as tools for the synthesis of new hybrid molecules and biomaterials. Appl Microbiol Biotechnol 82:605?624
- Nwanze PI, Jatto W, Oranusi S, Josiah SJ (2006) Proximate analysis of Lentinus squarrosulus (Mont.) Singer and Psathyrella atroumbonata Pegler. Afr J Biotech 5(4):366?368
- Ozcan S. and M. Johnston (1999). Function and regulation of yeast hexose transporters. Microbio Molbiol. 63:554-569.
- Rothschild, N., C. Novotny, V. Sasek and C.G. Dosoretz (2002). Ligninolytic enzymes of the fungus Irpex lacteus (Polyporus tulipiferae) : isolation and characterization of lignin peroxidase. Microb. Technol. 31: 627-633
- Saparrat, M. C. N., M. J. Martinez, H. A. Tournier, M. N. Cabello and A. M.isolated from different substrata. World . J. Microbiol. Biotechnol. 16: 799-803
- Steffen, K. T., M. Hofrichter and A. Hatakka (2000). Mineralisation of super (14) Clabelledsynthetic lignin and ligninolytic enzyme activities of litter-decomposing basidiomycetousfungi . Microbio. 54: 819-825
- Sedmak JJ, Grossberg SE (1977) A rapid, sensitive and versatile assay for protein using coomassie brilliant blue G-250. Anal Biochem 79:544?552
- Shin K-S, Kim YH, Lim J-S (2005) Purification and characterization of manganese peroxidase of the white-rot fungus Irpex lacteus. J Microbiol 43(6):503?509
How to Cite This Article
Neha Thakur, Astha Tripathi, Sukrit Sagar, Pardeep Kumar, Nagina Devi, Shaveta, Isha Sharma, Monika and Jyoti Sharma. (2017); ESTIMATION OF EXTRACELLULAR LIGNINOLYTIC ENZYMES FROM WILD AURICULERIA POLYTRICHA, HELVELLA SP. AND MORCHELLA SP., Int. J. of Adv. Res., 5 (10), 968-974, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/5612
Corresponding Author
This work is licensed under a Creative Commons Attribution 4.0 International License.





