Sequencing of 16S rRNA Gene and Phylogenetic Analysis of Pseudomonas aeruginosa isolated from well water in Mosul city/Iraq.
- Msc. student, Dep. of Biology, College of Science, University of Mosul / Iraq.
- Professor, Dep. of Biology, College of Science, University of Mosul / Iraq.
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Abstract
Pseudomonas aeruginosa is a gram negative bacterium isolated from well water. The isolate was cultured in a MacConkey agar and nutrient agar at 37?C for 24 hours. For species identification, Pseudomonas like organisms creates lot of problems when identified with the help of morphological and biochemical characters. However, sequencing of 16S rRNA region is a suitable technique for species identification. The amplified product of 16S rRNA was submitted to NCBI database. Amplification of 16S rRNA gene region, and new sets of primer pairs were designed by NCBI database search tool. To study phylogenetic relationship between various strains of Pseudomonas aeruginosa have often been based on sequencing of 16S rRNA gene region. Distance tree was constructed to find out genetic similarity between the organisms. Hence gene sequencing of 16S rRNA region was a suitable technique to identify Pseudomonas aeruginosa at molecular level.
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Elaaf M. Jassim Alsamarraui and Amera M. Alrawi. (2017); Sequencing of 16S rRNA Gene and Phylogenetic Analysis of Pseudomonas aeruginosa isolated from well water in Mosul city/Iraq., International Journal of Advanced Research (IJAR), 5 (10), 1846-1850, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/5710
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