Detection of van A gene of Vancomycin resistant Staphylococcus aureus by PCR technique

The pattern of minimum inhibitory concentration of S.aureus isolates to vancomycin , was determined; The results revealed that 2 S.aureus isolates(33,56) were vancomycin resistant, the MIC was 32-64 ?g\ml respectively ,these two isolates were multi-drug resistant , while MIC of 8 S.aureus isolates was vancomycin-intermediate between (8-16 ?g\ml). Deoxyribo nucleic acid (DNA) extraction from S.ureus isolates was done manually, the isolates were subjected to polymerase chain reaction (PCR) technique in monoplex pattern to amplify resistant incoding gene : the vanA gene ; results by this study showed that 2 S. aureus isolated gave the implicone size (1032 base pair) of the vanA gene , the results of MIC &PCR was similar.