Molecular and Serological detection of the most common Brucella species infecting bovine and human in Egypt

Brucellosis is one of the most important diseases affecting human and animals in most of the developing countries including Egypt. Rapid and accurate diagnosis is fundamental for control and eradication of brucellosis. The present work aimed to determine of the most common brucella species biotypes infecting human and animals by using serological and multiplex PCR . A total of 170 samples (90 serum samples from male and 80 serum samples from female as well as the same number from male and female whole blood samples with ( EDTA anticoagulant) at different age and 190 animals sera and the same number of samples whole blood with EDTA anticoagulant collected from (135 cow & 55 buffaloes ) through the period between (February 2011 to March 2013 ) in Egypt . Their sera were tested by ELISA and SAT. DNA was extracted and examined by multiplex PCR ( MPCR) involving specific primers for Brucella species (B.SPP), Brucella melitensis (B. melitensis) and Brucella abortus (B. abortus) based on IS711 in the brucella chromosome. Brucellosis was confirmed in patient by ELISA IgM was ( 23.3%) in male and ( 17.5%) in female, brucella IgG in male was (37.7%) and in female was (26.25%) and by SAT was (25.5%) in male and (22.5%) in female at El Fayoum Governorate respectively. Comparing serological tests with MPCR in human (male and female ) serum and blood samples gave total positive of ELISA IgM (20.58 %) , ELISA IgG, (32.32 ), SAT ( 24.11% ) and MPCR (32.9 %). Also ELISA (18.5 % ), SAT (14.8) and MPCR (21.48% ) in cow and ELISA (14.5% ), SAT (12.7%) and MPCR (5.5%) in buffaloes in the same region. The present study proved that multiplex PCR assay is a rapid , sensitive and accurate technique for diagnosis of B.SPP biotype compared to ELSA IgM, IgG and SAT. However ELISA was simple, accurate, rapid, does not require specialized training or equipment and economical for the detection of Brucella antibody.