15Nov 2017

A STUDY OF SERUM AND FOLLICULAR FLUID C. REACTIVE PROTEIN AND ANTISPERM ANTIBODY AS PREDICTIVE MARKERS FOR HUMAN OOCYTE AND EMBRYO QUALITY AND ICSI OUTCOME.

  • High Institute of Infertility Diagnosis and ART, Al-Nahrain University, Baghdad / Iraq.
  • Collage of Medicine, Al-Nahrain University, Baghdad / Iraq.
  • Kamal Al-Samarrai Hospital for Infertility Treatment/ BAGHDAD-IRAQ.
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Background: C. reactive protein (CRP) is an acute phase reactant. Its level increases in infection, inflammation and following hormonal stimulation. While antisperm antibodies (ASA) cause immunological infertility through their affect on motility and capability of fertilization of spermatozoa which may negatively affect embryo development & implantation Objectives: To determine the predictive value of C. reactive protein (CRP) & Antisperm antibodies (ASA) detection in serum and follicular fluid for assessment of oocyte quality, embryo quality and ICSI outcome in patients using different stimulation protocols. Materials and Methods: Fifty eight (58) infertile women undergoing intra-cytoplasmic sperm injection cycles with an age of (21-41) years old were included in this study. C. reactive protein was measured in serum and follicular fluid by using agglutination and serial dilution method. Also an estimation of antisperm antibodies (ASA) levels in serum and follicular fluid was done by using ELISA. Results: The results of this study showed a significant negative correlation between the C. reactive protein& Anti-sperm antibodies levels in serum and follicular fluid with oocyte and embryo quality in all patients. Eighteen women (31%) achieved pregnancy (pregnant group), while forty women (69%) failed to achieve pregnancy (non-pregnant group). A significant difference was found in the concentration of C. reactive protein and antisperm antibodies in both serum and follicular fluid (P<0.05) between pregnant and non-pregnant groups. Conclusion: Serum and Follicular Fluid C. reactive protein and Anti-sperm antibodies are good predictors for oocyte quality, embryo quality and pregnancy rate in women undergoing IVF/ICSI cycles.

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[Mohammad Oda Selman, Mufedah Ali Jawad, Ahmed R. Abu Raghif, Mustafa H.O. Hussein, Muayad Sraibet Abbood and Qays A. M. Al-khafaji. (2017); A STUDY OF SERUM AND FOLLICULAR FLUID C. REACTIVE PROTEIN AND ANTISPERM ANTIBODY AS PREDICTIVE MARKERS FOR HUMAN OOCYTE AND EMBRYO QUALITY AND ICSI OUTCOME. Int. J. of Adv. Res. 5 (Nov). 488-494] (ISSN 2320-5407). www.journalijar.com

Mohammad Oda Selman
High Institute of Infertility Diagnosis and ART, Al-Nahrain University, Baghdad / Iraq.

DOI:

Article DOI: 10.21474/IJAR01/5794      
DOI URL: https://dx.doi.org/10.21474/IJAR01/5794

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