In vitro propagation method of Withania somnifera by tissue culture technique.

The experiment has been conducted to achieve a method for producing multiple shoots from sterilized shoot-tips and obtaining a lot of seedlings of ever green shrub Withania somnifera, family Solanaceae as well as studying the effect of different culture media composition on shoots formation. Our results revealed that the level of 2.0 mg/l BAP+0.1 mg/l NAA increased the number of W. somnifera shoot/explant and the increasing of BAP concentration to 3.0 mg/l significantly decreased number of shoot/explant to about 50%. On the other hand the tallest shoot length which derived from the treatment of 2.0 mg/l BAP+ 1.0 mg/l NAA was a significant increment compared with other concentrations of growth regulators. The concentration of 1.0 mg/l Kin+0.1 mg/l NAA gave the shortest shoots compared with the other treatments including the control and the difference was significant.