Production, purification and characterization of alkaline protease from Trichoderma viride N9 by using agro industrial wastes under solid state fermentation

The production of enzymes by bioprocesses is a good value added to agro industry residues. A comparative study was carried out on the production of protease using agro industrial wastes (Wheat bran, rice bran, green gram husk and black gram husk) as substrates in solid-state fermentation (SSF) by Trichoderma viride N9. Among the all tested varieties, wheat bran produced the highest activity as 59.6 U/mg of protein, while black gram produced lowest protease as 56.1 U/mg of protein under solid state fermentation conditions. The purified alkaline protease from Trichoderma viride N9 obtained from all samples appeared as a single protein band with a molecular weight of 23KDa. The protease production from waste substrates could be commercially used in detergents and leather industry.