THE EFFECT OF CAMEL MILK WHEY PROTEINS ON LACTIC ACID BACTERIA ISOLATED FROM CAMEL AND COW MILKS IN EGYPT: A COMPARATIVE STUDY.

  • Animal Production Division, Desert Research Center, Cairo, Egypt.
  • Dairy Technology Department, Animal Production Research Institute, Agricultural Research Center, Giza, Egypt.
  • Department of Natural Resources,Instituteof African Research and Studies, Cairo University, Cairo, Egypt.
  • Abstract
  • Keywords
  • References
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  • Corresponding Author

Camel milk also has valuable nutritional properties as it contains a high proportion of antibacterial substances that found naturally in camel milk or derived by lactic acid bacteria .This study was carried out to evaluate the effect of camel\'s milk whey on lactic acid bacteria (LAB) isolated from cows\' and camels\' milk. Fifty-eight lactic acid bacteria species (41, 17 isolates were obtained from camel milk and cow milk respectively) were isolated from 14 camel\'s milk samples and 5 samples of raw cow\'s milk and 2 samples of zabady were collected from different regions in Egypt and Sudan, followed by pre-identification using phenotypic and genotypic methods using rep-PCR techniques. Then All LAB strains isolated from both camel\'s and cow\'s milk were examined to grow at constant concentration of whey camel\'s milk and its ability to grow was determined as optical density during 24 hours. Based on phenotypic and genotypic identification, Lactobacilli isolates were identified as: Lb rhamnosus (8.6%), Lb.fermentum (10.3%), Lb.acidophilus (8.6%), Lb. delbrueckii subsp. lactis( 7%), Lb.plantarum (13.8%). Among cocci isolates, Lc. lactis subsp.lactis were 13.8%, Lc.garvieae were 1.7%, Lc. lactis subsp. cremoris were 1.7%, Enterococcus faecium were 27.6% and Streptococcus thermophilus were 5.2%. Whereas, 1.7% of isolates were identified as Bidobacterium animalis. All LAB that isolated from camel?s milk showed a good ability to grow at the concentration of whey 20% compared with those isolated from cow?s milk.


  1. Abdalla, A.A. (2007). A study on the fermented camel's milk. Ph.D. Thesis, University of Al-Gezira, Sudan.
  2. Abdel Rahman IE; Dirar and Osman MA, ( 2009) . ''Microbiological and chemical changes and sensory evaluation of camel milk fermented by selected bacterial starter cultures''. African Journal of Food Science, 3, 398-405
  3. Agrawal, R. P., Beniwal, R.; Sharma, S.; Kochar, D. K.; Tuteja, F. C. ; Ghorui, S. K.; and Sahani, M. S.( 2005). "Effect of raw camel's milk in type 1 diabetic patients: 1 year randomized study". Journal of Camel Practice Research. 12(1):27-35.
  4. Akhmetsadykova S.H; Baubekova, A.; Konuspayeva, G.; Akhmetsadykov, N.; Faye, B, and Loiseau, G. (2015) African Journal of Food Science and Technology ((ISSN: 2141-5455) Vol. 6(3) pp. 84-88
  5. Asres, A.and Yusuf, M. "Traditional Consumption, Therapeutic Value and Its Derived Dairy Products of Dromedary Camel (Camelus Dromedaries) Milk in Somali Regional State, Eastern Ethiopia" . Advances in Life Science and Technology. vol. 26, 2014, pp. 48:52.
  6. Ashmaig, A., Hasan, A. and El Gaali, E. (2009). "Identification of lactic acid bacteria isolated from traditional fermented camel's milk (Gariss)" African Journal of mirociological Research. 3 (8), 451:457.
  7. Carr, FJ., Chill D., and Maida, N. The lactic acid bacteria: a literature survey. Critical. Reviews in Microbiology (2002).? 28 : 281-370.
  8. Dalmasso, , Prestoz,? S., Rigobello, V., and Demarigny Y (2008). Evolution of the raw cow milk microflora especially lactococci enterococci leuconostocs and lactobacilli over a successive 12 day milking regime. Int. J. Dairy Sci. 3: 117-130
  9. de Man, J.C., Rogosa, T. M. and Sharpe, E. (1960). A medium for the cultivation of lactobacilli. J. Appl. Bact. 23: 130.
  10. Estifanos, H., Guesh,T., Kebede, A. and Menkir, S. (2016). Characterization of Lactic Acid Bacteria from Camel Milk and their Technological Properties to Use as a Starter Culture. East African Journal of Sciences 10 (1) 49-60.
  11. Farah, Z., (1993): Composition and characteristics of camel milk, Journal of Dairy Research 60, 603-626.
  12. Hamama, A. and Bayi, M. (1991): Composition and microbiological profile of two Moroccan traditional dairy products: RaibandJben; Journal of the Society of Dairy Technology.44 (4):118-120
  13. Hamad, S. H. (2012). Factors Affecting the Growth of Microorganisms in Food. In Progress in Food Preservation. Edited by R. Bhat, . Alias and G. Paliyath. JohnWiley and Sons, Ltd. 405-428.
  14. Hamed, E and El Attar, A. (2013) "Identification and Some Probiotic Potential of Lactic Acid Bacteria Isolated From Egyptian Camels Milk" Life Science Journal , 10 (1), 1953: 1961.
  15. Hayek,S. and. Ibrahim, A. (2013). ''Current Limitations and Challenges with Lactic Acid Bacteria: A Review''. Food and Nutrition Sciences. 4, 73-87
  16. Kandler, O. and Weiss, N. (1986). Genus Lactobacillus. Beijerink 1901, 212AL . In: Sneath, P. A., Mair, N. S., Holt, J. G. (Eds.), Brgys Manual of Systematic Bacteriology, Vol. 2. Wiliams and Wilkins, Batimore, MD, pp. 1209.
  17. Khitam, (2003) "Camel milk plasma may help produce anti-microbial?? vaccine"? Gulf News Al Nisr Publishing LLC
  18. Kŕĭ?ovă, J., ?panovă, A., and Rittich, B. (2008) ''RAPD and rep-PCR fingerprinting for the characteristization of Bifidobacterium species'' Folia Microbiologica . 53 (2), 99-104.
  19. Isono, Y., Shingu, I. and Shimizu, S. (1994): Identification and characterization of lactic acid bacteria isolated from Masai fermented milk in northern Tanzania. Bioscience-Biotechnology and Biochemistry. 58, (4): 660-664.
  20. Jans, Christoph, V. (2011). "Biodiversity of lactic acid bacteria in raw camel milk products of East Africa including genomic and functional characterization OF predominant lactose-adapted Streptococcus infantarius subsp. Infantarius" Doctoral Thesis, ETH Zurich, Research collection.
  21. Jrad, Z.; El Hatmi, H.; Fguirl, I.; Arroum, S.; Assadi, M.; and Khorchan,i T. (2013). "Antibacterial activity of Lactic acid bacteria isolated from Tunisian came milk" African Journal of Microbiology Research. 7 (12), 1002:1008.
  22. Kappeler SR.; Heuberger C.; Farah Z.; and Puhan Z (2004). ''Expression of thepeptidoglycan recognition protein, PGRP, in the lactating mammary gland'. Journal of Dairy. Science. 87: 2660?2668
  23. Khedid, K.; Faid, M.; Mokhtari. A.; Soulaymani, A.; and Zinedine, A. (2009). Characterization of lactic acid bacteria isolated from the one humped camel milk produced in Morocco. Microbiological Research 164: 81-91.
  24. Lechiancole, T., Ricciardi, A. and Parente, E. (2002 ).?Optimization of Media and Fermentation Conditions for the Growth of Lactobacillus sakei,? Annals of Microbiology, Vol. 52, pp. 257-274.
  25. Mertin, p.; pochart , P.; Flourie, B. ; Pellier, P.; and Santos , L .; and Desieg, M. (2001) . Effects of chronic ingestion of fermented diary product containing Lactobacillus acidophilus and Bifidobacteriumbifidum on metabolic activates of colonic flora in humans . American .Journal of Clinical Nutrition. 52 :685- 688.
  26. Mathara, J.M., Schillinger, U., Kutima, P.M., Mbugua, S.K. andHolzapfel, W.H. (2004): Isolation, identification and characterization of the dominant microorganisms of kule naoto: the Maasai traditional fermented milk in Kenya. International Journal of Food Microbiology. 94: (3, 1): 269-278.
  27. Muna, A. and Adel, M. (2014) "Isolation of Lactobacillus Strains with propiotic potentials from camel's milk " African Journal of Microbiology Research, 8 (15), 1645-1655.
  28. Mohammed, M.; Abd El-Aziz, H.; Omran, N.; Anwar, S.; Awad, S. and El-Soda, M.? (2009) " Rep-PCR characterization and biochemical selection of lactic acid bacteria isolated from the Delta area of Egypt". International Journal of Food Microbiology . 128( 3), 417 : 423
  29. Nakamura, T.; Sugai, M.; Ozawa, A.N.; Ariga, H.; Koaze, H. and Kiiyukia, C. (1999)' Microbiological properties of Maziwa lala, a Kenyan traditional fermented milk of Masai community; Milk Science. 48, (1): 9-14.
  30. Pfeiler, E. A., and Klaenhammer, T. R. (2007). The genomics of lactic acid bacteria. Trends in Microbiology, 15, 546-553.
  31. Rahman, N.; Xiaohong C; Meiqin, F.and Mingsheng, D. (2009). Characterization of the dominant microflora in naturally fermented camel milk shubat. World J. Microbiol. Biotechnol. 25: 1941?1946
  32. Renner, E., Schaafsma, G. and Scott, K. J. (1989) "Micronutrients in Milk and Milk-based Food Products" . Elsevier Applied Science, London, UK, pp. 1-70.
  33. Reiter, B., (1985.) The biological significance of the non-immunoglobulin protective proteins in milk: Lysozyme, lactoferrin, lactoperoxidase, xanthine oxidase. Developments in Dairy Chemistry 3:281-336.
  34. Savadogo A.; Ouattara, CAT.; Savadogo PW.; Ouattara AS.; Barro, N.; and Traor?, AS. (2004). ''Microorganisms Involved in Fulani Traditional Fermented Milk in Burkina Faso.'' Pakistan Journal of Nutrition.3,134-139
  35. . Singh, R. S. Ghorui, K. and Sahani. M. S. ( 2006).''Camel's milk: Properties and Processing Potential'', In: M. S. Sahani (Ed.) pp. 59-73. The Indian Camel. NRCC, Bikaner.
  36. Singh, M. B.;Lakshminarayana, J. and Fotedar. R. ( 2009). Nutritional status of adult population of Raika community in Jodhupur desert district of Rajasthan. J. Hum. Ecol. 26(2):77-80.
  37. Song, D.; Ibrahim, S.; and Hayek, S. (2012). ''Recent Application of Probiotics in Food and Agricultural Science??. In: E. C. Rigobelo, Ed., Probiotics, InTech, Manhattan, 2012, pp. 1-34.
  38. Watabe, J.; Ikeda, N.; Mizutani, J.; Sato, N.; Jin, s.; Hirai, T.; and Ariga, H. (1998): Comparison of microbiological and chemical characteristics among types of traditionally fermented milk in Inner Mongolia in China and Calpis sour milk (Sannyuu); Milk Science. 47, (1): 1-8.
  39. Yoneya, T.; Nakajima, H.; Shimizu, K.; Miyamoto, T. and Kataoka, K (1999): ''Isolation and characterization of lactic acid bacteria from Ergo (Traditional Ethiopian fermented milk)''.Milk Science. 48, (2): 65-71.

[Mariam Mohammed, Marwa H.El-Gendy, Aida S. Salem and Wafai Z. A. Mikhail. (2018); THE EFFECT OF CAMEL MILK WHEY PROTEINS ON LACTIC ACID BACTERIA ISOLATED FROM CAMEL AND COW MILKS IN EGYPT: A COMPARATIVE STUDY. Int. J. of Adv. Res. 6 (Feb). 986-998] (ISSN 2320-5407). www.journalijar.com


Mariam Mohammed
Mohammed M.

DOI:


Article DOI: 10.21474/IJAR01/6519      
DOI URL: https://dx.doi.org/10.21474/IJAR01/6519