Expression of u-msf GFP cloning vector of DH 5? strain for Production of Green Fluorescent protein in one liter bioreactor

The present investigations were carried out for the optimization and production of GFP in Escherichia coli DH-5? strain. Based on the results obtained through shake flask experimentation, process is forwarded to produce GFP using 1 liter bioreactor (Spectrochem Laboratories Hyderabad). Around 2 to 3 colonies of Escherichia coli contain GFP gene was isolated and inoculated on to 650 ml (1 Liter Bioreactor) of LB broth that contain 1000µl of ampicillin. After 12 hr the growth rate was suddenly increased to 1.4 which is measured at 600 nm. Exponential and steady state phases were lasting in between 11.25 hours to 29 hours and found maximum cell density 4.2 with the significant production of protein with 9.6 mg/ml. The doubling time during the exponential phase was noticed approximately is 4.81 hour. Cell density remained almost constant in between 29 to 31.0 hrs.