27May 2018

COMPARISON STUDY BETWEEN BASE AND FREE LIPASE ENZYME CATALYSED TRANSESTERIFICATION IN THE SYNTHESIS OF BIODIESEL FROM CARICA PAPAYA SEED OIL.

  • Department of Biotechnology, Manipal International University Nilai, Negeri Sembilan, Malaysia.
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The main objective of the present study was to evaluate the suitability of different catalyst usage in the production of biodiesel using papaya seed as the feedstock. Non-seasonal papaya seeds were bought from local market, oven-dried and oil was extracted with n-hexane solvent. Physiochemical analysis was done to the seed oil. Transesterification was carried out using methanol to oil in the ratio of 9:1 with base and lipase catalysts. Papaya seed yielded 34% of oil which makes it an attractive feed stock in the biodiesel synthesis. Extracted seed oil also had an Iodine value of 64.98 ? 0.97 (g I/100g), Saponification Value of 14.00 ? 9.90 (mg KOH/g), Free fatty acid content of 1.18 ? 0.41(%) and Unsaponifiable matter of 4.55(%). Biodiesel catalysed from base catalyst gave a yield of 90% while lipase catalyst gave 50% of biodiesel. Synthesised biodiesel samples did not show any corrosion to copper stripes. Moreover, combustion energy released and cetane number determined for biodiesel synthesised using base catalysed transesterification and lipase catalysed transesterification were 1255.8J/g and 1172.08J/g and 79 and 76 respectively. Base synthesised biodiesel was analysed using GC-MS analysis and it was determined that the major fatty acid methyl esters present in the sample were Palmitic acid, Trans-13-Octadenoic acid and Oleic acid in the percentages of 73.9%, 14.4% and 10.9% respectively. Hence, the results shows that this local agricultural waste has good potential as biodiesel feedstock and base catalyst is a better catalyst than free lipase enzymes in the biodiesel production.

  1. Agunbiade, F. O., & Adewole, T. A. (2014). Methanolysis of Carica papaya Seed Oil for Production of Biodiesel. Journal of Fuels, 2014 (Article ID 904076), 6. https://doi.org/10.1155/2014/904076
  2. Ali, O. M., Mamat, R., Abdullah, N. R., Abdullah, A. A., Khoerunnisa, F., & Sardjono, R. E. (2016). Effects of different chemical additives on biodiesel fuel properties and engine performance. A comparison review. MATEC Web of Conferences, 38, 3002. https://doi.org/10.1051/matecconf/20163803002
  3. Aravind, G., Bhowmik, D., Duraivel, S., & Harish, G. (2013). Traditional and Medicinal Uses of Carica papaya. Journal of Medicinal Plants Studies, 1(1), 7?1
  4. Barab?s, I., & Todoruţ, I.-A. (n.d.). Biodiesel Quality, Standards and Properties.
  5. Chai, M., Tu, Q., Lu, M., & Yang, Y. J. (2014). Esterification pretreatment of free fatty acid in biodiesel production , from laboratory to industry. Fuel Processing Technology, 125, 106?113.
  6. Facchini, D. A., Vici, A. C., Pereira, M. G., de Oliveira, M. F., Batista, A. C. F., Vieira, A. T ., Silva, T. A., Jorge, J. A., & . Polizeli, M. L. T. M. (2016). Useful methodology to select lipase-catalyzed transesterification aiming biodiesel application. Brazilian Journal of Biosystems Engineering, 10(1), 01-13
  7. ISI Handbook of Food Analysis (Part XIII)-1984 Page 67/ IUPAC 2.201(1979) /IS: 548 (Part 1) ? 1964, Methods of Sampling and Test for Oils and Fats/ ISO 660:1996 Determination of acid value and acidity)
  8. ISI Handbook of Food Analysis (Part XIII)-1984, page 67 /AOAC 17th edn, 2000, Official method 933.08, Residue (unsaponifiable) of oils and fats
  9. Javidialesaadi, , & Raeissi, S. (2013). Biodiesel Production from High Free Fatty Acid-Content Oils: Experimental Investigation of the Pretreatment Step. APCBEE Procedia, 5(August), 474?478. https://doi.org/10.1016/j.apcbee.2013.05.080
  10. Jesikha, M. (2012). Fatty Acid Methyl Esters Characteristic and Esterification of Some Vegetable Oils for Production of Biodiesel. RESEARCH INVENTY: International Journal of Engineering and Science, 50-53
  11. Lee, W. J., Lee, M. H., & Su, N. W. (2011). Characteristics of papaya seed oils obtained by extrusion-expelling processes. Journal of the Science of Food and Agriculture, 91(13), 2348?2354. https://doi.org/10.1002/jsfa.4466
  12. Malacrida, C. R., Kimura, M., & Jorge, N. (2011). Characterization of a high oleic oil extracted from papaya (Carica papaya L .) seeds. Food Science and Technology Research, 31(4), 929?934. https://doi.org/10.1590/S0101-20612011000400016
  13. Medina, D.L.C. J., Guti?rrez,V. G., & Garc?a, H. (2003). PAPAYA Post-harvest Operations. INPho, Post-Harvest Compendium, 71.
  14. Mathur,A. (2016). Extraction and studies on saponification values of some non edible seed oils from arid zone of rajasthan , (3), 2393?2395.
  15. Ranganathan, S. V., Narasimhan, S. L., & Muthukumar, K. (2008). An overview of enzymatic production of biodiesel. Bioresource Technology, 99(10), 3975?3981. https://doi.org/10.1016/j.biortech.2007.04.060
  16. Ribeiro, B. D., Castro, A. M. De, Alice, M., Coelho, Z., & Guimar, D. M. (2011). Production and Use of Lipases in Bioenergy : A Review from the Feedstocks to Biodiesel Production, 2011. https://doi.org/10.4061/2011/615803
  17. Samaram, S., Mirhosseini, H., Tan, C. P., & Ghazali, H. M. (2013). Ultrasound-assisted extraction (UAE) and solvent extraction of papaya seed oil: Yield, fatty acid composition and triacylglycerol profile. Molecules, 18(10), 12474?12487. https://doi.org/10.3390/molecules181012474
  18. Singh, B., Korstad, J., & Sharma, Y. C. (2012). A critical review on corrosion of compression ignition (CI) engine parts by biodiesel and biodiesel blends and its inhibition. Renewable and Sustainable Energy Reviews, 16(5), 3401?3408. https://doi.org/10.1016/j.rser.2012.02.042
  19. Subenthiran, S., Choon, T. C., Cheong, K. C., Thayan, R., Teck, M. B., Muniandy, P. K., Ismail, Z. (2013). Carica papaya Leaves Juice Significantly Accelerates the Rate of Increase in Platelet Count among Patients with Dengue Fever and Dengue Haemorrhagic Fever. Evidence-Based Complementary and Alternative Medicine, 2013, 1?7. https://doi.org/10.1155/2013/616737
  20. Syed, h. M., Kunte, s. P., & Salve, r. V. (2012). Extraction and characterization of papaya, International journal of applied, physical and bio-chemistry research (IJAPBCR) 2(1), 33?43.
  21. Wong, C.S., & Othman, R.(2015). Biodiesel Production by Enzymatic Transesterification of Papaya Seed Oil and Rambutan Seed Oil. Melaka, Malaysia: International Journal of Engineering and Technology, 6(6), 2773-2777. Retrieved from http://www.enggjournals.com/ijet/docs/IJET14-06-06-115.pdf
  22. Zahir, E., Saeed, R., Hameed, M. A., & Yousuf, A. (2017). Study of physicochemical properties of edible oil and evaluation of frying oil quality by Fourier Transform-Infrared (FT-IR) Spectroscopy. Arabian Journal of Chemistry, 10(June 2014), S3870?S3876. https://doi.org/10.1016/j.arabjc.2014.05.025
  23. Zhang, Q., Wu, J., Ma, P., Cai, J., & Zhang, Y. (2015). Acid Value Determination and Pre-Esterification of Crude Euphorbia lathyris L . Oil, (May), 70

[Zahrathul Zilfa Niaz and Shamala Marimuthu. (2018); COMPARISON STUDY BETWEEN BASE AND FREE LIPASE ENZYME CATALYSED TRANSESTERIFICATION IN THE SYNTHESIS OF BIODIESEL FROM CARICA PAPAYA SEED OIL. Int. J. of Adv. Res. 6 (May). 1023-1029] (ISSN 2320-5407). www.journalijar.com

SHAMALA MARIMUTHU
Department of Biotechnology, Manipal International University

DOI:

Article DOI: 10.21474/IJAR01/7125      
DOI URL: https://dx.doi.org/10.21474/IJAR01/7125

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