SIMULTANEOUS DETERMINATION OF CLOTRIMAZOLE AND TINIDAZOLE IN TABLET AND CREAM BY HPTLC

The present work describes a normal phase HPTLC method for simultaneous determination of clotrimazole and tinidazole in tablet and cream. The developed method is more sensitive than the reported method. Chromatographic separation was carried out on aluminum-backed silica gel 60 GF254 TLC plates with mobile phase comprising of toluene: ethyl acetate: methanol: triethyl amine (5.5:1.0:1.0:0.1, v/v). The validated calibration ranges were 200-700 ng/spot (r=0.9960 and 0.9960 by height and area respectively) and 500-1750 ng/spot (r=0.9990 and 0.9975 by height and area respectively) for clotrimazole and tinidazole respectively. Quantitation was achieved with UV detection at ?=220 nm. The method was validated in terms of accuracy, precision and specificity. The method has been successfully applied to pharmaceutical formulations. Interference was not observed from the tablet and cream formulations excipients.