Vol. 6 (04) pp. 449-455 DOI: 10.21474/IJAR01/6878

A STUDY OF BIOFILM FORMATION & EXTENDED SPECTRUM-β-LACTAMASES PRODUCTION IN PSEUDOMONAS AERUGINOSA AT A TERTIARY CARE HOSPITAL IN HARYANA.

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  • Department of Microbiology, Pt. B. D. Sharma PGIMS, Rohtak, Haryana.
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Abstract

Introduction: Emergence of antibiotic resistance and biofilm formation among the nosocomial pathogens is of serious concern due to recurrence and chronicity of infections. The production of the extended spectrum-β-lactamases and their spread among bacterial pathogens is a matter of concern with regard to the future of antimicrobial therapy. Antimicrobial resistance is an innate feature of bacterial biofilms. Biofilm formation is higher in ESBL producing and MDR strains. The present study was undertaken with the aim to determine antibiotic resistance pattern of the isolates, to find prevalence of ESBL among isolates and their association with biofilm production. Material and Methods: The study was carried out in the tertiary care hospital for the period of 1yr, from November 2014 to October 2015. A total of 100 isolates of P. aeruginosa from various clinical specimens were taken and identified using standard microbiological procedures. The isolates were subjected to antimicrobial susceptibility testing and ESBL production as per CLSI method. Biofilm formation was achieved according to the Tissue Culture Plate (TCP) assay. Results: Out of these 100 isolates, 58 were multi drug resistant and 20 were extreme drug resistant. Forty nine (49.0%) isolates were ESBL producers, while 51(51.0%) isolates were non-ESBL producers. Seventy-eight isolates produced biofilm, out of which, 43 were found in ESBL producers and 34 in non-ESBL producers. Conclusion: ESBL and biofilm producing nosocomial pathogens are of significant concern as they can cause chronic relapses and dissemination of drug resistant isolates. Both ESBL and biofilm production in the isolates further increase the morbidity and mortality in hospitalized patients. Hence it is necessary to detect the biofilm forming bacteria, conduct surveillance of the antibiotic susceptibility and device effective infection control measures.

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How to Cite This Article

Alfia Alim, Aparna Parmar and Uma Chaudhary. (2018); A STUDY OF BIOFILM FORMATION & EXTENDED SPECTRUM-β-LACTAMASES PRODUCTION IN PSEUDOMONAS AERUGINOSA AT A TERTIARY CARE HOSPITAL IN HARYANA., International Journal of Advanced Research (IJAR), 6 (04), 449-455, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/6878

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Alfia Alim
M.D Microbiologist

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References

  1. Church D, Elsayed S, Reid O, Winston B, Lindsay R. Burn wound infections. Clin Microbiol Rev. 2006;19(2):403?34.
  2. Pirnay JP, De Vos D, Cochez C, Bilocq F, Pirson J, Struelens M, et al. Molecular epidemiology of Pseudomonas aeruginosa colo?nization in a burn unit: persistence of a multidrug-resistant clone and a silver sulfadiazine-resistant clone. J Clin Microbiol. 2003;41(3):1192?202.
  3. Falagas ME, Koletsi PK, Bliziotis IA. The diversity of definitions of multidrug-resistant (MDR) and pandrug-resistant (PDR) Aci?netobacter baumannii and Pseudomonas aeruginosa. J Med Mi?crobiol. 2006;55(Pt 12):1619?29.
  4. Van Delden C, Iglewski BH. Cell-to-cell signaling and Pseudomo?nas aeruginosa Emerg Infect Dis. 1998;4(4):551?60.
  5. Nicas TI, Iglewski BH. The contribution of exoproducts to virulence of Pseudomonas aeruginosa. Can J Microbiol. 1985;31(4):387?92.
  6. Carpentier B, Cerf O. Biofilms and their consequences, with particular reference to
    1. hygiene in food industry. J Appl Bacteriol. 1993; 75: 499-511.
  7. Sauer K, Camper AK, Ehrlich GD, Costerton JW, Davies DG. Pseudomonas aeruginosa displays multiple phenotypes during development as a biofilm. J Bacteriol 2002;184: 1140-54.
  8. Mitov I, Strateva T, Markova B. Prevalence of virulence genes among bulgarian nosocomial and cystic fibrosis isolates of pseu?domonas aeruginosa. Braz J Microbiol. 2010;41(3):588?95.
  9. O'Toole GA. Microtiter dish biofilm formation assay. J Vis Exp. 2011;(47)
  10. Nikokar I, Tishayar A, Flakiyan Z, Alijani K, Rehana-Banisaeed S, Hossinpour M, et al. Antibiotic resistance and frequency of class 1 integrons among Pseudomonas aeruginosa, isolated from burn patients in Guilan, Iran. Iran J Microbiol. 2013;5(1):36?41.
  11. Collee JG, Mles RB, Watt B. Tests for identification of bacteria. In : Collee JG, Fraser AG, Marmon BP, Simmons A, editors. Mackie and McCartney Practical Medical Microbiology. 14th New York: Churchill Livingstone; 1996.p.131-49.
  12. Elmer W Koneman, Stephen D Allen, William M Jenda et. al. Color Atlas and Textbook of Diagnostic Microbiology, 6th edition 2006. Publishers J.B. Lippincot Company.
  13. Christensen GD, Simpson WA, Younger JA, Baddour LM, Barrett FF, Melton DM. Adherence of cogulase negative Staphylococi to plastic tissue cultures: a quantitative model for the adherence of staphylococci to medical devices.?J. Clin. Microbiol.1985;22:996?1006.
  14. Mathur T, Singhal S, Khan S, Upadhyay DJ, Fatma T, Rattan A. Detection of biofilm formation among the clinical isolates of staphylococci: An evaluation of three different screening methods. I J. Med. Microbiol.2006,24(1)25-9.
  15. Costerson JW, Stewart PS, Greenberg EP. Bacterial biofilms: A common cause of persistant infections. Sciences 1999;284:1318-22.
  16. O?Tolle GA, Kolter R. Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: A genetic analysis. Mol Microbiol 1998;28:449-61
  17. Bakshi R, Walia G, Shikha J. Prevalence of extended spectrum β-lactamases in multidrug resistant strains of gram negative Bacilli. J Acad Indus Res. 2013;1:558-60.
  18. Goel V, Hogade SA, Karadesai SG. Prevalence of extended spectrum beta-lactamases, AmpC beta-lactamase, metallo-beta-lactamase producing Pseudomonas aeruginosa and Acinetobacter baumannii in an intensive care unit in a tertiary care hospital. J Sci Soc 2013; 40:28-31.
  19. Lim KT, Yasin RM, Yeo CC, Puthucheary SD, Balan G, Maning N et al. Genetic fingerprinting and antimicrobial susceptibility profiles of Pseudomonas aeroginosa hospital isolates in Malaysia. J Microbiol Immunol Infect 2009;42:197-209
  20. Jacoby AmpC β lactamases. Clin Microbiol Rev 2009;22(1):161-82.
  21. Tavajjohi Z, Moniri R, Khorshidi A. Detection and characterization of multidrug resistance and extended-spectrum-beta-lactamase-producing (ESBLS) Pseudomonas aeruginosa isolates in teaching hospital. Af J Microbiol Res. 2011;5(20):3223-8
  22. Pagani L, Mantengoli E, Migliavacca R, Nucleo E, Pollini S, Spalla M et al. Multifocal detection of multidrug resistant Pseudomonas aeruginosa producing the per-1 extended-spectrum β-lactamase in northern italy. J Clin Microbiol. 2004;42:2523-9.
  23. Mansouri S, Norouzi F, Moradi M, Nakhaee N. Comparison of virulence factors among clinical isolates ofPseudomonas aeruginosa?producing and non-producing extended spectrum β-lactamases.?Curr Res Bacteriol.?2011; 4:85?93.
  24. Selvakumar BN, Jasmine R. Antibiotic susceptibility of ESBL-producing urinary isolates at a tertiary care hospital in Tiruchirappalli, South India. J. Med. Sci. 2007;7:443-6.
  25. Livermore DM, Woodford N. The beta-lactamase threat in Enterobacteriaceae, Pseudomonas and Acinetobacter. Trends Microbiol. 2006;14:413-20.
  26. Shivani Saxena, Banerjee G, Garg R, Singh M. Biofilm Formation in Pseudomonas aeruginosa isolates from LRTI. J Clin Diag Research. 2014;8(5): DC09-DC11
  27. Khalil MA, Ibrahim Sonbol F, Mohamed AF, Ali SS. Comparative study of virulence factors among ESβL-producing and non-producing Pseudomonas aeruginosa clinical isolates. Turk J Med Sci 2015;45(1): 60-9.
  28. Choy MH, Stapleton F, Willcox MD, Zhu H. Comparison of virulence factors in Pseudomonas aeruginosa strains isolated from contact lens-and non-contact lens-related keratitis. J Med Microbiol. 2008;57(12):1539-46.
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