24Jun 2018

THE EFFECT OF STARVATION ON HISTOLOGICAL AND IMMUNOHISTOCHEMICAL FEATURES OF THYMIC EPITHELIAL RETICULAR CELLS, USING CD326 MARKER EXPRESSION, IN NEWBORN MICE.

  • Department of Human Anatomy, Section of Histology& Embryology, University of AL-Nahrain, College of Medicine, Baghdad, Iraq.
  • Abstract
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  • Corresponding Author

The thymus gland serves a role in the training and development of T-lymphocytes (T cell), cells of thymic gland, have vital effect in proliferation, differentiation and maturation of T- lymphocytes. They secrete certain biomarker proteins on their cell surfaces called epithelial cells adhesion molecules (EPCAM). This study aimed to analyze the effect of starvation in newborn mice on anthropometric and immunohistochemical changes of reticular epithelial cell (RECs) on growth and development of thymus gland using anti EPCAM receptor 2. The study design enrolled two groups; control groups consist of (20) newborn are sacrificed according to (1, 7, 14 and 30 days) each groups (5) litters and starvation groups consist of (15) newborn are sacrificed according to (7, 14 and 30 days) each group (5) litters and ; the thymus glands are processed histologically and immunhistochemically to demonstrate reticular epithelial cells reactivity of EPCAM by applying Aperio Algorithm software. The EPCAM are expressed and present on cell membranes of RECs are decreased with the progress of starvation involving the whole layers of thymic tissue (subcapsular, cortical, cortico-medullary and medullary areas). This indicates that EPCAM is important in signaling, proliferation and differentiation of the progenitor migrating immature thymocytes so that be graduated as mature CD4 and CD8 T lymphocytes. It has prognostic value in thymic tissue development, regeneration and normal epithelial homeostasis.


  1. Condie, N. R. M. B. G., and B. G. Condie.( 2010). Transcriptional regulation of thymus organogenesis and thymic epithelial cell differentiation. Prog. Biol. Transl. Sci. 92: 103?120.
  2. Rode, I., Martins, V. C., K?blbeck, G., Maltry, N., Tessmer, C., & Rodewald, H. R. (2015). Foxn1 protein expression in the developing, aging, and regenerating thymus.?The Journal of Immunology,?195(12), 5678-5687.
  3. Moore, K. L., Dalley, A. F., & Agur, A. M. (2013). Clinically oriented anatomy. Lippincott Williams & Wilkins (161 )( 191-1170).
  4. Zuckerwise, Lisa C., Li, L., & Copel, J. A. (2018). In Obstetric Imaging: Fetal Diagnosis and Care (Second Edition)(pp. 25-28). e1.
  5. Itoi, M., Kawamoto, H., Katsura, Y., & Amagai, T. (2001). Two distinct steps of immigration of hematopoietic progenitors into the early thymus anlage. International immunology, 13(9), 1203-1211.
  6. Gordon, J., & Manley, N. R. (2011). Mechanisms of thymus organogenesis and morphogenesis.?Development,?138(18), 3865-3878.
  7. Junqueira, L. C., Mescher, A. L & Carneiro, J. (2013). Basic histologytext and atlas. London: McGraw Hill, 65-85,287-297.
  8. Rizzo, Donald. C. (2015). Fundamentals of anatomy and physiology. Cengage Learning , p ( 371? ) , (358- 515)
  9. Schiaffino, S., Mammucari, C., & Sandri, M. (2008). The role of autophagy in neonatal tissues: just a response to amino acid starvation?. Autophagy, 4(5), 727-730.
  10. Anastasiadis, K., & Ratnatunga, C. (Eds.). (2014). The thymus gland: diagnosis and surgical Springer Science & Business Media., P ( 5- 112 ) .
  11. Danneman, P. J., Suckow, M. A., & Brayton, C. (2012). The laboratory mouse. CRC Press. 5, 21-45.
  12. Wang, T., Hung, C. C., & Randall, D. J. (2006). The comparative physiology of food deprivation: from feast to famine. Annu. Rev. , 68, 223-251.
  13. Duerr, J. S. (2006). Immunohistochemistry.
  14. Dabbs, David J.?(2018)Diagnostic Immunohistochemistry E-Book: Theranostic and Genomic Applications. Elsevier Health Sciences.
  15. Patriarca C, Macchi RM, Marschner AK, Mellstedt H.. Epithelial cell adhesion molecule expression (CD326) in cancer: a short review.. Cancer Treat Rev. 2012; 38(1):68-75.
  16. Farr, A. N. D. R. E. W., Nelson, A. N. D. R. E. W., Truex, J. I. L. L., & Hosier, S. U. Z. A. N. N. E. (1991). Epithelial heterogeneity in the murine thymus: a cell surface glycoprotein expressed by subcapsular and medullary epithelium. Journal of Histochemistry & Cytochemistry, 39(5), 645-653.
  17. Hussan MT, Khan MZI, Lucky NS (2009) Immunohistochemical study of the postnatal development of lymphoid tissues and mucosa of broilers. Bangl J Vet Med 7(1): 253-258.
  18. Treuting, P. M., Dintzis, S. M., Liggitt, D., & Frevert, C. W. (Eds.). (2011). Comparative anatomy and histology: a mouse and human atlas (expert consult). Academic Press.
  19. Bancroft, J. D., Floyd, A. D., & Suvarna, S. K. (2018). Bancroft's Theory and Practice of Histological Techniques,53, 83, 93, 105-121, 433-517.
  20. Babbie, E., Wagner III, W. E., & Zaino, J. (2018).?Adventures in social research: Data analysis using IBM? SPSS? Statistics. Sage Publications.
  21. Schmelzer, E., & Reid, L. M. (2008). EpCAM expression in normal, non-pathological tissues. Frontiers in bioscience: a journal and virtual library, 13, 3096-3100.
  22. Trzpis, M., McLaughlin, P. M., de Leij, L. M., & Harmsen, M. C. (2007). Epithelial cell adhesion molecule: more than a carcinoma marker and adhesion molecule. The American journal of pathology, 171(2), 386-395.
  23. Schnell, U., Cirulli, V., & Giepmans, B. N. (2013). EpCAM: structure and function in health and disease. Biochimica et Biophysica Acta (BBA)-Biomembranes, 1828(8), 1989-2001.
  24. Reza, J. N., & Ritter, M. A. (1994). Differential expression of adhesion molecules within the human thymus. Clinical and Developmental Immunology, 4(1), 55-64.
  25. Sun, L., Luo, H., Li, H., & Zhao, Y. (2013). Thymic epithelial cell development and differentiation: cellular and molecular regulation. Protein & cell, 4(5), 342-355.
  26. Spizzo, G., Fong, D., Wurm, M., Ensinger, C., Obrist, P., Hofer, C., ... & Went, P. (2011). EPCAM expression in primary tumour tissues and metastases: an immunohistochemical analysis. Journal of clinical pathology, 64(5), 415-420.

[Huda Hussein Abd Fayr, Haider A. Jaafar and Hussein A. Jarallah. (2018); THE EFFECT OF STARVATION ON HISTOLOGICAL AND IMMUNOHISTOCHEMICAL FEATURES OF THYMIC EPITHELIAL RETICULAR CELLS, USING CD326 MARKER EXPRESSION, IN NEWBORN MICE. Int. J. of Adv. Res. 6 (Jun). 1072-1080] (ISSN 2320-5407). www.journalijar.com


huda
Department of Human Anatomy, Section of Histology& Embryology, University of AL-Nahrain, College of Medicine, Baghdad, Iraq.

DOI:


Article DOI: 10.21474/IJAR01/7312      
DOI URL: https://dx.doi.org/10.21474/IJAR01/7312