DETERMINATION OF SERUM MATRIX METALLOPROTEINASE 2 AND 9 (MMP-2) (MMP-9) IN BREAST CANCER INVASION AND METASTASIS.

  • Department of Medical Lab Technology, Health and Medical Technology College, Southern Technical University. Basrah, Iraq.
  • Department of Biology, College of Science, Basrah University.
  • Medical College, Basrah University.
Crossref Cited-by Linking logo

  • Abstract
  • Keywords
  • References
  • How to Cite This Article
  • Corresponding Author

Breast cancer is the most frequently diagnosed cancer and the leading cause of cancer death among women worldwide. Matrix metalloproteinases (MMPs) are a family of enzymes implicated in the degradation and remodeling of extracellular matrix and in vascularization. The current study, was aimed to determine the effect of gelatinizes in breast cancer progression by the measurement the levels of MMP-2 and MMP-9 in serum of breast tumor by ELISA. The present study involved 80 samples including 60 patients of breast tumor, which were selected from Medical Centre for Oncology, and 20 as control(normal subject),which select fromsouthern technical university from south region of Iraq in Basrah city during the period from October 2017 to February 2018.The results showed that measurements of MMP-2 and MMP-9 in the serum of the women with malignant tumor were highly significant (P≤0.01) compared with benign and control, regarding the grade of cancer subjects, stage and lymph node metastasis.This conclude the present of a correlation of the serological measurement of MMP-2 and MMP-9 in patients with breast cancer and metastasis.

  1. Jemal A, Tiwari RC, Murray T, Ghafoor A, Samueis A, Ward E, Feuer EJ &Thum MJ (2004). Cancer statistics. CA Cancer J Clin 54, 9?29.
  2. Kessenbrock K, Plaks V &Werb Z (2010). Matrix metalloproteinases: regulators of the tumor microenviroment. Cell 141, 52?67.
  3. A. Walker, ?Breast,? in General and Systemic Pathology, J. C. E. Underwood, Ed., pp. 467?492, Churchill Livingstone, 4th edition, 2005.
  4. Page-McCaw A, Ewald AJ &Werb Z (2007). Matrix metalloproteinases and the regulation of tissue remodelling. Nat Rev Mol Cell Biol 8, 221?233.
  5. Nagase H, Visse R & Murphy G (2006). Structure and function of matrix metalloproteinases and TIMPs. Cardiovasc Res 69, 562?573.
  6. Roy R, Yang J & Moses AM (2009). Matrix Metalloproteinases as novel biomarkers and potential therapeutic targets in human cancer. J ClinOncol 27, 5287?5297.
  7. Xu, Y. Wang, Z. Chen, M. D. Sternlicht, M. Hidalgo, and B. Steffensen, ?Matrix metalloproteinase-2 contributes to cancer cell migration on collagen,? Cancer Research, vol. 65, no. 1, pp. 130?136, 2005.
  8. Kohrmann, U. Kammerer, M. Kapp, J. Dietl, and J. Anacker (2009). ? ?Expression of matrix metalloproteinases (MMPs) in primary human breast cancer and breast cancer cell lines: new findings and review of the literature,? BMC Cancer, vol. 9, article 188.
  9. Lebeau, A. G. Nerlich, U. Sauer, R. Lichtinghagen, and U. Lohrs (1999). ?Tissue distribution of major matrix metalloproteinases ? and their transcripts in human breast carcinomas,? Anticancer Research, vol. 19, no. 5, pp. 4257?4264.
  10. J. Duffy, T. M. Maguire, A. Hill, E. McDermott, and N. O?Higgins (2000). ?Metalloproteinases: role in breast carcinogenesis, invasion and metastasis,? Breast Cancer Research, vol. 2, no. 4, pp. 252?257.
  11. Shiomi and Y. Okada, ?MT1-MMP and MMP-7 (2003).in invasion and metastasis of human cancers,? Cancer and Metastasis Reviews, vol. 22, no. 2-3, pp. 145?152.
  12. Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, et al. (2015) Cancer Incidence and mortality Worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer 136: E359-E386.
  13. Ferlay J, Soerjomataram I, Ervik M, et al. GLOBOCAN (2012) Cancer incidence and mortality worldwide: IARC, Lyon, France. International Agency for Research on Cancer;.
  14. Habib OS, Al-Ali JK, Al-Wiswasi MK, et al (2007). Cancer Registration in Basrah 2005. Preliminary results. Asian Pac J Cancer Prev, 8, 187-90
  15. Cancer in Basrah: Epidemiological analysis of incident cancer 2005-2008.Basrah Cancer Research Group. Dar Al-Kutub for Publication, 2009.
  16. Al-Badri K, Ajeel NA (1998). Study of the risk factor of female breast cancer in Basrah Governorate. Med J Basrah, 16, 21-38.
  17. Habib OS, Al-Diab JMA, Mohsin A, et al (2010). Experience and outcome of population-based cancer registration in Basrah-Southern Iraq in four years (2005-2008). Asian Pac J Cancer Prev, 11, 51-4
  18. Chen, Y. Breast Cancer. http://www.nlm.nih.gov/medlineplus/ ency/article/000913.htm 2012.
  19. Stamenkovic I: Matrix metalloproteinases in tumor invasion and metastasis. Semin Cancer Biol 10: 415-433, 2000.
  20. Liotta LA, Stetler-Stevenson WG (1991) Tumor invasion and metastasis: an imbalance of positive and negative regulation. Cancer Res 51: 5054s-5059s.
  21. Stetler-Stevenson WG: Dynamics of matrix turnover during pathologic remodeling of the extracellular matrix. Am J Pathol 148: 1345?1350, 1996
  22. Remacle AG, Noel A, Duggan C, McDermott E, O'Higgins N, Foidart JM, Duffy MJ: Assay of matrix metalloproteinases types 1, 2, 3 and 9 in breast cancer. Br J Cancer 77(6): 926? 931, 1998Google Scholar
  23. Heppner KJ, Matrisian LM, Jensen RA, Rodgers WH: Expression of most matrix metalloproteinase family members in breast cancer represents a tumor-induced host response. Am J Pathol 149(1): 273?282, 1996
  24. Basset P, Bellocq JP, Anglard P, Chenard MP, Lefebvre O, Noel A, Okada A, Rouyer N, Santavicca M, Stoll I, Wolf C, Rio MC: Stromelysin-3 and other stromelysins in breast cancer: importance of epithelial-stromal interactions during tumor progression. Breast Cancer Res Treat 83: 353?367, 1996.
  25. Ranuncolo SM, Armanasco E, Cresta C, Bal De Kier Joffe E and Puricelli L: Plasma MMP-9 (92 kDa-MMP) activity is useful in the follow-up and in the assessment of prognosis in breast cancer patients. Int J Cancer 106: 745-751, 2003.
  26. Huhtala P, Chow LT, Eyggvason K. Structure of the human type IV collagenase gene. J BiolChem 1990;265:11077.
  27. Heo, D. S.; Choi, H.; Yeom, M. Y.; Joo. B.; Jeong Oh, S. S. (2014). Serum levels of matrix metalloproteinase-9 predict lymph node metastasis in breast cancer patients. 1567-1572.
  28. Noah, A. M.; Rajaa, M. F.; Nahi, Y. Y.; Mohamed, E. M. (2015). Matrix Metalloproteinases MMP2 and MMP9 Expression in Stages II-III Breast Cancer in Iraqi Women .2449-1810.
  29. Sandra, R.; Gordana, K.; Vladimir, J.; Katarina, K.; Marina, N.; Kristina, G. (2013). Values of MMP2 and MMP9 in tumor tissues of basil-like breast cancer patients. Cell. Biochem. Biophys. 68 (1):143-152.

Rawan Hamid Jarrah, Dhamia Kasim Suker, Khairallah AS Mohammed and Rafid Adel Abood. (2019); DETERMINATION OF SERUM MATRIX METALLOPROTEINASE 2 AND 9 (MMP-2) (MMP-9) IN BREAST CANCER INVASION AND METASTASIS., Int. J. of Adv. Res., 7 (01), 806-810, ISSN 2320-5407. DOI URL: https://dx.doi.org/10.21474/IJAR01/8391

Rawan Hamid Jarrah
Iraq

DOI:

Article DOI: 10.21474/IJAR01/8391      
DOI URL: https://dx.doi.org/10.21474/IJAR01/8391

Download Full Paper

Download PDF No. of Downloads: 30 | No. of Views: 135