12Apr 2019

EVALUATION DE LEFFET PROTECTEUR DU LAIT DE CHEVRE FERMENTE PAR LE KEFIR ET ENRICHI A LA CAROUBE CONTRE LULCERE GASTRIQUE INDUIT PAR LETHANOL CHEZ LE RAT.

  • Laboratoire dEcologie et de Technologie Microbienne, Institut National des Sciences Appliquees et de Technologie, Boulevard de la terre, BP 676 1080 Tunis, Tunisie.
  • Laboratoire de Physiologie Fonctionnelle et Valorisation des Bioressources-Institut Superieur de Biotechnologie de Beja, B.P. 382?9000, Beja, Universite de Jendouba, Tunisie.
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La caroube ou fruit de caroubier est traditionnellement utilis?e contre les plaies et l?ulc?re gastro-duod?nal. Le k?fir du lait est consomm? pour ces actions b?n?fiques sur la flore intestinale et le transit gastro-intestinal. Le but de cette ?tude est d??valuer l?activit? antiulc?reuse du lait de ch?vre ferment? par le k?fir et enrichi ? la caroube chez le rat. Pour cela, les boissons test?es (extrait aqueux de caroube, la boisson de k?fir ou le m?lange) ont ?t? administr?es par voie intra-gastrique durant deux semaines. La famotidine ou mol?cule de r?f?rence a ?t? utilis?e comme le t?moin positif ? la dose de 10mg/kg et le groupe non trait? a re?u de l?eau distill?e ? 10mL/kg. Le dernier jour, une heure apr?s, 1ml d??thanol (80%) a ?t? administr? aux animaux pour provoquer l?ulc?re pendant deux heures. Ensuite, les rats ont ?t? sacrifi?s. L?estomac de chaque rat a ?t? ouvert pour ?valuer les ulc?res. L?indice d?ulc?re a ?t? exprim? et le pourcentage de protection a ?t? calcul?. Les alt?rations associ?es ont ?t? mesur?es dans les surnageant par des m?thodes biochimiques. A 200mg/kg, l'extrait aqueux de caroube a induit une protection de la muqueuse de 53%, contre 32% pour le k?fir seul ? la dose de 20 mL/kg. Alors que, le m?lange kefir-caroube (20mL/kg) a montr? une action pr?ventive plus importante de 81%. Cette puissante protection est accompagn?e par une augmentation du taux de prot?ines et une r?gulation des m?diateurs cellulaires. La mise au point d?un bio-m?dicament contre l?ulc?re gastrique ? base d?un m?lange de caroube et de k?fir pourrait ?tre envisageable.

  1. Arawwawala, L.D.A.M., Arambewela, L.S.R .et Ratnasooriya, WD. (2014). Effet gastroprotectrice de piper betle linn feuilles cultiv?es au sri Lanka. Article de recherche originale: exp?rimental, 5(1) : 38-42.
  2. Corsi, L., Avallone, R., Cosenza, F., Farina, F., Baraldi, C., Baraldi, M. (2002). Antiproliferative effects of Ceratonia siliqua L. on mouse hepatocellular carcinoma cell line. Fitoterapia, 73(7)?: 674- 684.
  3. Dingeon B, Ferry JP, Roullet A.(1975) Automatic assay of blood sugar by Trinder's method. Ann Biol Clin. 33:3?13.
  4. Diniz, R., Garla, L., Schneedorf, J., and Carvalho, J. C. (2003). Study of anti-inflammatory activity of Tibetan mushroom, a symbiotic culture of bacteria and fungi encapsulated into a polysaccharide matrix.? Res.47?: 49?52.
  5. De Souza Almeida ES, Filho VC, Niero R, Classen BK, Balogun SO, De Oliveira MDT (2011). Pharmacological mechanisms underlying the anti-ulcer activity of methanol extract and canthin-6-one of Simaba ferruginea A. St-Hil. in animal models 12;134(3):630-6. doi: 10.1016/j.jep.2011.01.009.
  6. Doronicheva, N., Yasui, H., Sakurai, H. (2007). Chemical structure-dependent differential effects of flavonoids on the catalase activity as evaluated by a chemiluminescent method. Biological and Pharmaceutical Bulletin, 30(2) : 213- 217.
  7. Durazzo, A., Turfani, V., Narducci, V., Azzini, E., Maiani, G., & Carcea, M. (2014). Nutritional characterisation and bioactive components of commercial carobs flours. Food Chemistry, 153?: 109-113.
  8. Farnworth, E.R., 2005. Kefir?A Complex Probiotic. Food Science Technology Bulletin: Functional Foods 13 May.
  9. Farnworth ER, Mainville I (2008) Kefir - A Fermented Milk Product. In: Farnworth, E. R. (2th ed.), Handbook of Fermented Functional Foods (2 ed). CRC Press Taylor & Francis Group, Boca Raton, London, New York, p. 89-127.
  10. Gao, J., Gu, F., Ruan, H., Chen, Q., He, J., and He, G. (2013). Induction of apoptosis of gastric cancer cells SGC7901 in vitro by a cell-free fraction of Tibetan kefir.? Dairy J.30?: 14?18
  11. Garrote G.L., Abraham A. G. & De Antoni G. L. (2001). Chemical and microbial characterisation of kefir grains. J. Dairy Res. 68?: 639-652
  12. Hollander D, Taranawski A, Krause WJ, Gergely H. (1985). Protective effect of sucralfate against alcohol-induced gastric mucosal injury in the rat. Gastroenterol. 88?: 366?374.
  13. Ioannis J. Stavrou, Atalanti Christou, Constantina P. Kapnissi-Christodoulou. Polyphenols in carobs 52018). A review on their composition, antioxidant capacity and cytotoxic effects, and health impact Ioannis J. Stavrou, Atalanti Christou, Constantina P. Kapnissi-Christodoulou. Food chemistry 269?: 355-374.
  14. Koroleva NS (1988). Kefir and kumy starters. IDF Bulletin 1988; 227: 96-100.
  15. Lam EK, Tai EK, Koo MW, Wong HP, et al., Enhancement of gastric mucosal integrity by Lactobacillus rhamnosus. GG. Life Sci. 80 (2007) 2128?2136.
  16. Lee M, Ahn K, Kwon O, Kim M et al., (2007). Anti-inflammatory and anti-allergic effects of kefir in a mouse asthma model. Immunobiol. 212 647-654.
  17. Leardi A, Caraglia M, Selleri C, Pepe S, Pizzi C, Notaro R. 1998. Desferioxamine increases iron depletion and apoptosis induced by ara-C of human myeloid leukaemic cells. Br J Haematol.102:746?52.
  18. Loeb JH, Vandenplas Y, Wursch P, Guesry P (1989). Tannin-rich carob pod for the treatment of acute-onset diarrhea, J. Paediatr. Gastroenterol. Nutr. 8 (4) (1989) 480?485.
  19. Miller T A, Henagan JM, Indomethacin decreases resistance of gastric barrier to disruption by alcohol. Dig. Dis. Sci. 29 (1984) 141?149.
  20. Naghmouchi, S., Khouja, M. L., Romero, A., Tous, J., Boussaid, M. (2009). Tunisian carob (Ceratonia siliqua L.) populations: Morphological variability of pods and kernel. Scientia Horticulturae, 121(2), 125-130.
  21. Orhan YT,?Karag?zl? C,?Sarioğlu S,?Yilmaz O,?Murat N,?Gıdener S. (2012) A study on the protective activity of kefir against gastric ulcer. Turk J Gastroenterol.2012 Aug; 23(4):333-8.
  22. Prado M.R., Garcia L, Vandenberghe, Rodrigues, Castro, Soccol V. 2015 Milk kefir?: composition, microbila cultures, biological activities and related products. Frontiers in Microbiology. 6?: 1177.
  23. Pop C , Apostu S , Salanta L , Rotar AM, Sindic M , Mabon N , Socaciu C. 2014. Influence of Different Growth Conditions on the Kefir Grains Production, used in the Kefiran Synthesis . Bulletin UASVM Food Science and Technology 71(2)
  24. Quir?os, A., Hern?andez-Ledesma, M., Ramos, L., Recio, I. (2005). Angiotensin-converting enzyme inhibitory activity of peptides derived from caprine Kefir. Journal of Dairy Science, 88, 3480?3487.
  25. Reddy RS, Swapna LA, Ramesh T, Singh TR, (2011) Bacteria in oral health ? Probiotics and Prebiotics. A Review. Int J Biol Med Res. 2, 1226 -1233.
  26. Rtibi, K., Jabri, M. A., Selmi, S., Souli, A., Sebai, H., El-Benna, J., Amri, M., & Marzouki, L. (2015a). Carob pods (Ceratonia siliqua L.) inhibit human neutrophils myeloperoxidase and in vitro ROS-scavenging activity. Rsc Advances, 5(102), 84207-84215.
  27. Rtibi, K., Jabri, M. A., Selmi, S., Souli, A., Sebai, H., El-Benna, J., Amri, M., & Marzouki, L. (2015b). Gastroprotective effect of carob (Ceratonia siliqua) against ethanol-induced oxidative stress in rat. BMC Complementary and Alternative Medicine, 15(1), 292.
  28. Rtibi, K., Selmi, S., Grami, D., Amri, M., Eto, B., El-Benna, J., Sebai, H., & Marzouki, L. (2017). Chemical constituents and pharmacological actions of carob pods and leaves (Ceratonia siliqua L.) on the gastrointestinal tract: A review. Biomedicine and Pharmacotherapy, 93?: 522-528.
  29. Rtibi, K., Selmi, S., Grami, D., Saidani, K., Sebai, H., Amri, M., Eto, B., Marzouki, L. (2017). Ceratonia siliqua L. (immature carob bean) inhibits intestinal glucose absorption, improves glucose tolerance and protects against alloxan-induced diabetes in rat. Journal of the Science of Food and Agriculture, 97(8)?: 2664-2670
  30. Rejeb M N (1995). Le caroubier en Tunisie: Situations et perspectives d'am?lioration. Dans Quel avenir pour l'am?lioration des plantes? Edit. AUPELF-UREF. John Libbey Eurotext, Paris,79?85.
  31. Ribeiro AC, , Ribeiro SDA, 2010.? Specialty products made from goat milk. Small Ruminant Research 89, 225?233.
  32. Rizk, S., Maalouf, K., and Baydoun, E. (2009). The Antiproliferative effect of kefir cell-free fraction on HuT-102 malignant T lymphocytes.? Lymphoma Myeloma9?: 198?203.
  33. Romier B, Schneider Y J, Larondelle Y, During A, (2009). Dietary polyphenols can modulate the intestinal Inflammatory response, Nutr. Rev. 67?: 363?378.
  34. Satır, G., G?zel-Seydim, Z.B. (2015). Influence of Kefir fermentation on the bioactive substances of different breed goat milks. LWT - Food Science and Technology, 63 (2) 852-858.
  35. Sebai H, Souli A, Chehimi L, Rtibi K, Amri M, El-Benna J, Sakly M. (2013) In vitro and in vivo antioxidant properties of Tunisian carob (Ceratonia siliqua L.), Med. Plant Res. 7?: 85?90.
  36. Souli A, Sebai H, Chehimi L, Rtibi K, Tounsi H, Boubaker S, Sakly M, El-Benna J, Amri M (2015). Hepatoprotective effect of carob against acute ethanol induced oxidative stress in rat. Toxicology and Industrial Health. Sep;31(9):802-10. doi: 10.1177/0748233713475506.
  37. Selmi S, Rtibi K, Grami D, Sebai H, Marzouki L. (2017). Protective effects of orange (Citrus sinensis) peel aqueous extract and hesperidin on oxidative stress and peptic ulcer induced by alcohol in rat.14;16(1):152. doi: 10.1186/s12944-017-0546-y.
  38. Stern J, Lewis WH. 1957. The colorimetric estimation of calcium in serum with ocresolphthalein complexone. Clin Chim Acta. 2:576?80.
  39. Temiz M A, , Temur A, and ?elik I, Antioxidant Role and Hepatoprotective Effects of Carob (Ceratonia siliqua) Seeds against Ethanol-Induced Oxidative Stress in Rats, J.Food Nutr. Res., 2015, 3, 57?61.
  40. Verbeke, W (2005). Consumer acceptance of functional foods: Socio-demographic, cognitive and attitudinal determinants. Food Quality and Preference, 16: 45-57.
  41. Yoo JH, Lee JS, Lee YS, Ku S, Lee HJ. (2018). Protective effect of bovine milk against HCl and ethanol?induced gastric ulcer in mice J. Dairy Sci. 101:1?13
  42. Zubillaga M, Weill R, Postaire E, Goldman C, et al. (2001), Effect of probiotics and functional foods and their use in different diseases. Nutri Res. 21?: 569?557.

[Sana Mhir, Kais Rtibi, Lamia Ayed, Moktar Hamdi, Lamjed Marzouki and Hichem Sebai. (2019); EVALUATION DE LEFFET PROTECTEUR DU LAIT DE CHEVRE FERMENTE PAR LE KEFIR ET ENRICHI A LA CAROUBE CONTRE LULCERE GASTRIQUE INDUIT PAR LETHANOL CHEZ LE RAT. Int. J. of Adv. Res. 7 (Apr). 1019-1028] (ISSN 2320-5407). www.journalijar.com

Sana M\\\'HIR
Laboratoire d’Écologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie (INSAT), Boulevard de la terre, BP 676 1080 Tunis, Tunisie.

DOI:

Article DOI: 10.21474/IJAR01/8921      
DOI URL: https://dx.doi.org/10.21474/IJAR01/8921

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