10Jul 2019

INDIRECT ORGANOGENESIS IN SOLID AND LIQUID MEDIUMS FROM VITROPLANTS OF AGAVE TITANOTA.

  • Facultad de CienciasAgr?colas, Universidad VeracruzanaCircuito Gonzalo Aguirre Beltr?n s/n Zona Universitaria C.P. 91090 Xalapa, Veracruz, Mexico.
  • Facultad de Biolog?a, Universidad Veracruzana, Circuito Gonzalo Aguirre Beltr?n s/n Zona Universitaria C.P. 91090 Xalapa, Veracruz, M?xico.
  • Tecnol?gico Nacional de M?xico-Instituto Tecnol?gico de ?rsulo Galv?n, km 4.5 Carr. Cardel-Chachalacas, CP. 91667. ?rsulo Galv?n, Veracruz,Mexico.
Crossref Cited-by Linking logo

  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

In vitro cultivation is a useful technique for study, improvement and conservation of agaves. It was used as explants pieces of leaves and roots in MS solid and liquid mediums respectively, supplemented with Naphtaleneacetic Acid (NAA by its abbreviations in English) Benzylaminopurine (BAP by its abbreviations in English) and Dichlorophenoxyacetic Acid (2,4-D by its abbreviations in English) It was used concentrations of 0.0, 0.5, 1.0 and 2.0 mgl-1 to induce shoot formation. It was reported the development of callus within one month and half on solid cultivation, shoot formations were presented using 2.0 mgl-1 BAP and 0.5 mgl-1 of NAA having 60?1 shoots each container with hydration aspect, meanwhile in the combination 1.5 mgl -1 of BAP with 0.5 mgl-1 of NAA a lower number of shoots was reached, but they were healthier. The formation of shoots in liquid medium was presented within a month of cultivation, obtaining 6.6?1 shoots by healthy explants, even in the control treatment. Embryogenic callus was generated using BAP and 2,4-D.

  1. Abreu, E., Gonz?lez, G., Ort?z, R., Rodr?guez, P., Domech, R., Garriga, M. (2007). Aclimataci?n de plantas de henequ?nmicropropagadas (Agave fourcroydes). Estudio de diferentescondicionesen la etapa de vivero. Informaci?nT?cnicaEcon?micaAgraria 103 (2): 84-94.
  2. Arzate-Fern?ndez, A. M. y Mej?a-Franco, R. (2011). Capacidadembriog?nica de callosinducidosenejesembrionarioscig?ticos de Agave angustifolia RevistaFitotecnia Mexicana 34 (2): 101?106.
  3. Aureoles-Rodr?guez, F., Rodr?guez-de la O, J. L., Legaria-Solano, J. P., Sahag?n-Castellanos, J. y Pe?a-Ortega, M. G. (2008). Propagaci?n in vitro del ?maguey bruto? (Agave inaequidens Koch), unaespecieamenazada de inter?secon?mico. RevistaChapingoSerieHorticultura 14 (3): 263-269.
  4. Binh, T. L., Muoi, L. T., Oanh, H. T. K., Thang, T. D., Phong, D. Y. (1990). Rapid propagation of agave by invitro tissue culture. Plant Cell Tissue and Organ Culture 23: 67-70.
  5. Bordallo, P. N., Silva, D. H., Maria, J., Cruz, C. D., Fontes, E. P. (2004). Somaclonal variation on in vitro callus culture potato cultivars. HorticulturaBrasileira 22 (2): 300-304.
  6. Castellanos-Hern?ndez, O. A., Rodr?guez-Sahag?n, A., Rodr?guez-Dom?nguez, J. M., Rodr?guez-Garay, B. (2006). Organog?nesisindirecta y enraizamientoin vitro de Paulownia elongata. e-Gnosis 4: 1-12.
  7. Castro-Concha, L., Loyola-Vargas, V. M., Chan, J. L. y Robert, M. L. (1990). Glutamate dehydrogenase activity in normal and vitrified plants of Agave tequilana Weber propagated in vitro. Plant Cell Tissue and Organ Culture 22: 147?151.
  8. Enr?quez-del Valle, J. R., Estrada-Silias, A., Rodr?guez-Ort?z, G., Velasco-Velasco, V. A., Campos-?ngeles, G. V. (2013). Sustrato y dosis de fertirriegoen la aclimatizaci?n de vitroplantas de Agave americana oaxacensis. Revista de la Facultad de CienciasAgrarias. Universidad Nacional de Cuyo 45 (2): 341-348.
  9. Gonz?lez, G., Alem?n, S., Trujillo, R., Keb, M., Abreu, E., Barredo, F., Robert, M. L., Ortiz, R., Cornides, M. T. (2004). El cultivo in vitro comoalternativa de la recuperaci?nhenequenera (Agave fourcroydes). Biotecnolog?aAplicada 21 (1): 44-48.
  10. Groenewald, E. G., Wessels, D. C. J., Koeleman, A. (1977). Callus formation and subsequent plant regeneration from seed tissue of an Agave species (Agavaceae). ZPflazenphysiol 21 (4): 369-373.
  11. Madrigal-Lugo, R., Pineda-Estrada, F., Rodr?guez de la O, J. L. (1989). Agave. In: Ammirato, P. V., Evans, D. A., Sharp, W. R. y Bajaj, Y. P. S. (ed.) Handbook of Plant Cell Culture, Vol. 5. Ornamental Species. McGraw Hill Publ. Co. NewYork. pp: 206?227.
  12. Miguel-Luna, M. E., Enr?quez-del Valle, J. R., Velasco-Velasco, V. A., Villegas-Aparicio, Y., Carrillo-Rodr?guez, J. C., Rodr?guez-Ort?z, G. (2015). Composici?n del medio de cultivo y la incubaci?n para enraizarbrotes de Agave. Revista Mexicana de CienciasAgr?colas Pub. Esp. (6): 1151-1159.
  13. Morales-Gonz?lez, B., Nu?ez-Palenius, H. G. (2015). Micropropagaci?n de Agave tequilana Weber Variedad Azul ?El Coronel? enun Sistema de Inmersi?n Temporal (SIT). J?venesen la Ciencia 1 (2): 77-82.
  14. Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and biassays with tabacco tissue cultures. Physiology Plant 15: 473-497.
  15. Powers, D.E., Backhaus, R. A. (1989). In vitro propagation of Agave arizonica Gentry & Weber. Plant Cell, Tissue and Organ Culture 16: 57-60.
  16. Puente-Garza, C. A., Guti?rrez-Mora, A., Garc?a-Lara, S. (2015). Micropropagation of Agave salmiana: means to production of antioxidant and bioactive principles. Frontiers in Plant Science 6: 1-9.
  17. Robert, M. L., Herrera, J. L., Contreras, F., Scorer, K. N. (1987) In vitro propagation of Agave fourcroydes (Henequen). Plant Cell Tissue and Organ Culture 8: 37?48.
  18. Rodr?guez-Garay, B., Guti?rrez-Mora, A., Santacruz-Ruvalcaba, F. (1996). M?todos de propagaci?nbiotecnol?gicos y convencionalesenagav?ceas para zonas ?ridas. In: Izquierdo, J. y Palomino, G. (es). T?cnicasConvencionales y Biotecnol?gicas para la Propagaci?n de Plantas de Zonas ?ridas FAO Regional Office for the Latin American and Caribean Region. Santiago, Chile. pp: 57?86.
  19. S?nchez-Islas, A. V., Portillo, L. (2014). Proliferaci?n de brotesaxilaresentresespecies de Agave: efecto de sacarosa. BoletinNakari 25 (3): 47-49.
  20. Santacruz-Ruvalcaba, F., Portillo, L. (2009). Thin cell suspension layer as a new methodology for somatic embryogenesis in Agave tequilana Weber cultivar azul. Industrial crops and products 29: 609?614.
  21. Santacruz-Ruvalcaba, F., Guti?rrez-Pulido, H., Rodr?guez-Garay, B. (1999). Efficient in vitro propagation of Agave parrasana Plant Cell Tissue and Organ Culture 56: 163?167.
  22. Thomas, T. D. (2008). The role of activated charcoal in plant tissue culture. Biotechnology Advances 26: 618?631.
  23. Zhang, Y. M., Li, X., Chen, Z., Li, J. F., Lu, J. Y., Zhou, W. Z. (2013). Shoot organogenesis and plant regeneration in Agave hybrid, No. Scientia Horticulturae 161: 30?34.
 

[Lopez-Isabel B, Martinez-Hernandez Ma. de J, Nader-GarciaB. L and Adame- Garcia J. (2019); INDIRECT ORGANOGENESIS IN SOLID AND LIQUID MEDIUMS FROM VITROPLANTS OF AGAVE TITANOTA. Int. J. of Adv. Res. 7 (Jul). 940-945] (ISSN 2320-5407). www.journalijar.com

Martínez Hernandez Ma. de J.
Facultad de Ciencias Agricolas Universidad Veracruzana, México

DOI:

Article DOI: 10.21474/IJAR01/9448      
DOI URL: https://dx.doi.org/10.21474/IJAR01/9448

Download Full Paper

Download PDF No. of Downloads: 41 | No. of Views: 100