STUDY OF EFFECT OF pH ON ENZYME ACTIVITY OF PROTEASE PRODUCED BY BACTERIAL ISOLATES COLLECTED FROM SOIL SAMPLES OF LACHIWALA, DOON VALLEY

Proteases are typical enzymes that are being investigated for their potential usage in many areas of application, such as detergent, brewing meat, photography, leather and dairy.A large number of proteases have been purified and characterized from bacteria and few from fungi. Bacteria were isolated from the soil sample of Lachiwala, Doon Valley. Out of 64 bacterial pure cultures, 10 best protease producing microorganisms were isolated through activity zone methods. Protease activity was checked spectrophotometrically using azocasein dye as a substrate. 1 bacteria was selected from them. The maximum enzyme activity was obtained at near neutral pH (6.5).