Vol. 7 (11) pp. 361-366 DOI: 10.21474/IJAR01/10016

IN VITRO PROPAGATION OF CHRYSANTHEMUM (CHRYSANTHEMUM INDICUM L) WITH BENZYL AMINO PURINE AND NAPHTALENE ACETIC ACID SUPPLEMENTATION

  • Department of Agroecotechnology, Faculty of Agriculture, Pembangunan Panca Budi University, Medan, Indonesia
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Abstract

The in vitro propagation of the plant is a developed method in tissue culture. This method is developed to propagate the Chrysanthemum (Chrysanthemum indicum L). The purpose of this research was to find out the Benzyl Amino Purine (BAP) and Naphtalene Acetic Acid (NAA) supplementation towards the in vitro Chrysanthemum multiplication. The method used was the factorial Completely Randomized Design with two factors used, those were factor I: NAA consisted of 3 treatment levels: (N0) = Control without NAA. (N1) = 1ml/1 NAA. (N2) = 1.5 ml / L NAA. (N3) = 2 ml/L NAA. Factor II: growth regulator (BAP) consisted of 3 treatment levels (B0) = Without BAP Treatment as Control. (B1) = 1 ml /L BAP. (B2) = 1.5 ml / L BAP. (B3) = 2 ml/L BAP. The parameters observed were the number of roots, leaves and performed every week meanwhile the height, root length and the performance was observed after 8 weeks of propagation (8MST). The result shows, the addition of growth regulatory compounds (NAA and BAP) combinations took significant effect towards the parameters of the number of leaves meanwhile the plant height, number of roots and length of root were not significantly taken any effect

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How to Cite This Article

Rosmaria Girsang, Armaniar and Refnizuida (2019); IN VITRO PROPAGATION OF CHRYSANTHEMUM (CHRYSANTHEMUM INDICUM L) WITH BENZYL AMINO PURINE AND NAPHTALENE ACETIC ACID SUPPLEMENTATION, International Journal of Advanced Research (IJAR), 7 (11), 361-366, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/10016

Corresponding Author

Rosmaria Girsang
Department of Agroecotechnology, Faculty of Agriculture, Pembangunan Panca Budi University, Medan, Indonesia.

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References

  1. Abidin, Z. 1994. Fundamentals of Knowledge of Growth Regulatory Substances. Bandung: Space
  2. Altayani, A., Suaria, N., and Arjana, M, G, ... Length of cuttings and Rootone-F on growth and cuttings of chrysanthemum plants (Chrysanthemum, sp). Journal of Agro Echoes, 23 (2): 139-145
  3. Astutik 2010. The use of alar and BA (Benzyl adenine) in chrysanthemum tissue culture media. Buana Science 1 (10): 77-82
  4. Harjadi, S.S. 2009. Growth Regulatory Substances. Swadaya Spreader Publisher, Jakarta
  5. Karjadi, A.K. and Buchory, A. 2007. Effect of NAA and BAP on the Growth of the Garlic Meristem Network on Media B5. J. Hort 17 (3): 217-223.
  6. Kasli, 2009. Propagation Effort for Chrysanthemum (Chrysathemum sp) In Vitro. Straw 2. (3): 121-125
  7. Lestari, E.G. 2011 The Role of Growth Regulatory Substances in Plant Growth through Tissue Culture. AgroBiogen Journal 7 (1): 63-68
  8. Magdalena (2002) Magdalena, T.S., L. 2002. Effect of Cytokinins on in Vitro Morphogenesis and Ploidy of Pepper Capsicum annuum L. Electronic Journal of Polish Agricultural Universities, Agronomy-my, Vol. 5. Issues 1.
  9. Mahadi, I. 2016. The Effect of Giving Naphthalene Acetyl Acyd (NAA) and Kinetin Hormone in Bogor Pineapple (Ananas comosus (L.) Merr.) Tissue Culture cv. Queen. Bio-site, 2 (2): 27-31.
  10. Muhit A. 2007. Early Production Technique of Chrysanthemum (Chryantemum morpholium) Vegetative Seed. Agricultural Engineering Bulletin 12 (1). 14-18.
  11. Ni\'ah, F. Ratnasari, E. S, Luke. and Budipramana. 2012. The Effect of Giving Various Combinations of Sucrose and Kinetin Concentrations to the Induction of Potato Micro Tubers (Solanum Tuberosum L.) from In-Vitro Flower Granola. LanternBio, 1 (1): 41?48
  12. Rachmawati, D. L., Roviq, M and Islami, T. 2017. Atonic Composition and Coconut Water on the Growth of Bud Cane Chips (Saccharum officinarum L.). Journal of Plant Production, 5 (5): 851-859.
  13. Rahardja, P.C. 2007. Modern Plant Propagation Techniques. Swadaya Spreader Publisher, Jakarta.
  14. 2010. Chrysanthemum Innovation Business Opportunities Agricultural Research Agency. South Jakarta: Agro-innovation
  15. Su, Y., Liu, Y., and Zhang, X. 2011. Auxin cytoknin interaction regulate meristem development. Molecular Plant, 4 (4): 616-625.
  16. 2008. Effect of BAP and NAA Giving on the Growth of Chrysanthemum (Chrysantemum morpholium) in Tissue Culture. Biota X (3): 31-35.
  17. A, Santi. and N, Solvia. 2012. Effect of Myo-inositol and Active Charcoal on the Growth of Dendrobium Orchid Planlets in In Vitro Culture. J. Hort. 22 (3): 205-209, 2012
  18. 2003. Tissue Culture How to Efficiently Increase Plants. Agromedia Reader. Jakarta. 105 pages
  19. Yustina, 2004. In Vitro Propagation of Orchid Plants. Bandar Lampung: Publisher of the University of Lampung.