IN VITRO MULTIPLICATION OF BARANGAN BANANA (MUSA ACUMINTA L.)

  • Department of Agroecotechnology, Faculty of Agriculture, Pembangunan Panca Budi University, Medan, Indonesia
  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

The in vitro multiplication of Barangan Banana Plants (Musa acuminta L.) was carried out to determine the effectiveness of growth regulator cytokines and coconut water in multiplying barangan banana. The pupose of this study was to obtained the concentration of benzyl amino purine (BAP) and concentration of coconut water which triggers the multiplication of Barangan bananas by in vitro method. The plant material used was the barangan banana (Musa acuminta L.), BAP growth regulator and coconut water. This research experiment uses a completely non factorial randomized design consisting of 5 levels, namely B0 control, B1 0.5 ml, B2 1 ml, B3 10 ml, B4 20 ml. The parameters observed included the number of leaves, leaf length, number of roots, root length, plant height, number of tillers. The results showed that the treatment of BAP concentration and coconut water significantly affected (p <0.05) the number of roots and root length and did not significantly affect the number of leaves, plant height and also the number of Barangan Banana tillers


  1. Harahap, F. (2011). Plant Tissue Culture. Medan Unimed Press.
  2. Hasanah, A., Harahap F., and Silaban, R. (2018). The effect of Myo-Inositol and Indole Butyric Acid (IBA) on the formation of in vitro Pineapples root (Ananas comosus L.) From Sipahutar North Sumatra Indonesia, International Journal of Biological Research, 23-28
  3. Insani, H., Harahap, F., and Diningrat, S. (2018). The effect of coconut water and benzyl purine (BAP) in addition to the Growth of Pineapple From Sipahutar North Sumatra, Indonesia on In Vitro Condition. International Journal of Biological Research, 6 (2) (2018) 29-33
  4. Ira, D. (2010). Utilization of Banana and Coconut Water Waste as a Material for Culture of Orchid Tissue Tissue Month. BPPT. Jakarta.
  5. Jumjunidang, Nasir, Risaka, and Handayani H. (2005). In vitro testing techniques of banana resistance to fusarium wilt disease using toxin filtrate from fusarium oxysporum f. sp. Cubense. J. Hort 15 (2) 135-139
  6. Magdalena, T.S., L. Drozdowska., And M. Szota, (2002). Effect of cytokinins on in Vitro Morphogenesis and Ploidy of Pepper Capsicum annuum L. Electronic Journal of Polish Agricultural Universities Agronomy, 5 (1).
  7. Murdad, R., Latip, M.A., Aziz, Z.A and Ripin, R. (2010). Effects of Carbon Source and Potato Homogenete on In Vitro Growth and Development of Sabah?s Endangered Orchid Phalaenopsis gigantea. AsPac. J. Mol. Biol Biotechnol. 18 (1) 199-202
  8. Pamungkas, S. S. T. (2015). Influence of NAA and BAP Concentration on the Growth of Cavendish (Musa paradisiaca L.) Banana Explants Plant Explants Through In Vitro Culture. Gontor agrotech Science Journal, 2 (1) 31-45.
  9. Pisesha, P.A., Mattjik, N.A and Sukma, D. (2008). Effect of IAA, IBA, BAP, and Coconut Water Concentrations on the Establishment of Poinsettias (euphorbia pulcherrima wild et. Klotz) In Vitro. Paper Seminar for the Faculty of Agriculture, Bogor Institute of Agriculture, Bogor
  10. Rachmawati, D. L., Roviq, M and Islami, T. (2017). Composition of Atonic and Coconut Water in the Growth of Cane Bud Chips (Saccharum officinarum L.). Journal of Plant Production, 5 (5) 851-859
  11. Rotinsulu, Prihandarini and Sudiarso. (1998). Use of Coconut Water in Various Maturity Levels in Barangan Banana Tissue Culture Media (Musa paradisiaca sapientum L.). Research Report. Faculty of Agriculture, Sam Ratulangi University. Manado.
  12. Santoso, R. D and Sobir. (2013). Growth of Pineapple Plantlet (Ananas comosus L. Merr.) Of Smooth Cayanne Varieties from In Vitro Culture at Several BAP Concentrations and Plantlet Age. Bul. Agrohorti, 1 (1) 54-61
  13. Sunyoto, A. (2011). Cavendish Banana Cultivation is a lucrative side business. Berlian Media. Yogyakarta. Suyanti, Supriyadi, A. (2008). Banana Cultivation Processing and Market Peospek. Self-help Spreaders. Jakarta
  14. Smith R, (2013). Plant Tissue Culture Third Edition Techniques and Experiments. California Elsevier Inc.
  15. Seswita D. (2010). Use of Coconut Water as Growth Regulatory Substances in Curcuma xanthorrhiza Multipurpose Sprout (In Vitro). Littri's Journal, 16 (4) 135-140.
  16. Widiastuti, D. (2006). Preliminary Experiments of Tissue Culture in Chrysanthemum (Chrysanthemum morifolium) Plants. Bull Penel Hort. 15 (2).
  17. Widiastoety, D. (2014). Effect of Auxin and Cytokines on the Growth of Mokara Orchid Plantlet. J. Hort, 24 (3) 230-238.
  18. (2004). Tissue Culture How to Increase Plant Efficiency. Jakarta AgroMedia Reader.

[Armaniar, Rosmaria Girsang and Refnizuida (2019); IN VITRO MULTIPLICATION OF BARANGAN BANANA (MUSA ACUMINTA L.) Int. J. of Adv. Res. 7 (Nov). 367-371] (ISSN 2320-5407). www.journalijar.com


Armaniar
Department of Agroecotechnology, Faculty of Agriculture, Pembangunan Panca Budi University, Medan, Indonesia.

DOI:


Article DOI: 10.21474/IJAR01/10017      
DOI URL: https://dx.doi.org/10.21474/IJAR01/10017