Vol. 8 (04) pp. 107-112 DOI: 10.21474/IJAR01/10752

AMELIORATIVE ACTIVITY OF ASCORBIC ACID (AA) ON PARAQUAT (PQ) INDUCED SERUM BIOCHEMICAL ALTERATIONS IN EXPERIMENTAL RATS

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  • Post-graduate, Department of Veterinary Pathology, College of Veterinary Science, PVNRTVU, Rajendranagar, Hyderabad-500030, India.
  • Assistant Professor and Head, Department of Veterinary Pathology, College of Veterinary Science, PVNRTVU, Mamnoor, Warangal-506166, India.
  • Professor and Head, Department of Veterinary Pathology, College of Veterinary Science, PVNRTVU, Rajendranagar, Hyderabad-500030, India.
  • Professor, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, PVNRTVU, Rajendranagar, Hyderabad-500030, India.
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Abstract

The present study aimed to investigate the protective effect of ascorbic acid on paraquat induced serum biochemical alterations in experimental rats. Forty-eight (48) male albino Wistarrats were randomized into four (4) groups consisting of twelve (12) in each. Group 1- Control. Group 2 ? Paraquat at the rate of 40 mg/kg b.wt. Group 3 ? Ascorbic acid at the rate of 250 mg/kg b.wt. Group 4 ? Paraquat at the rate of 40 mg/kg b.wt + Ascorbic acid at the rate of 250 mg/kg b.wt. The treatment regimens were administered by oral gavage once daily for twenty-one (21) days. Significantly (P<0.05) increased activity of aspartate transaminase, alanine transaminase, alkaline phosphatase, blood urea nitrogen and serum creatinine and significantly (P<0.05) decreased activity of total protein was observed in group 2 on 7th and 21st day of experiment. Co-treatment with ascorbic acid showed a remarkable protection against the parameters investigated possibly via antioxidant defence mechanism.

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References

  1. Baltazar, T., Dinis‐Oliveira, R. J., Duarte, J. A., de Lourdes Bastos, M. and Carvalho, F. (2013): Paraquat research: do recent advances in limiting its toxicity make its use safer?. Br. J. Pharmacol., 168(1): 44-45.
  2. Chen, J. L., Dai, L., Zhang, P., Chen, W., Cai, G. S., Qi, X. W., Hu, M. Z., Du, B. and Pang, Q. F. (2015): Methylene blue attenuates acute liver injury induced by paraquat in rats.? Immunopharmacol.,28(1): 808-812.
  3. Costa, M. D., de Freitas, M. L., Dalmolin, L., Oliveira, L. P., Fleck, M. A., Pagliarini, P., Acker, C., Roman, S. S. and Brand?o, R. (2013): Diphenyl diselenide prevents hepatic alterations induced by paraquat in rats. Environ. Toxicol. Pharmacol., 36(3): 750-758.
  4. Das, B. K. and Mukherjee, S. C. (2000): Sublethal effect of quinalphos on selected blood parameters of Labeo rohita(Ham.) fingerlings.?Asian Fish. Sci.,?13(3): 225-233.
  5. Evers, W. D., Hook, J. B. and Bond, J. T. (1983): Paraquat concentration and renal function in mice fed purified and cereal-based diets.?Drug-nutrient Interactions.,?2(2): 95-104.
  6. Hemmati, A. A., Nazari, Z., Motlagh, M. E. and Goldasteh, S. (2002): The role of sodium cromolyn in treatment of paraquat-induced pulmonary fibrosis in rat. Pharmacol. Res., 46(3): 229-234.
  7. Hu, X., Shen, H., Wang, Y. and Zhao, M. (2017): Liver X receptor agonist TO901317 attenuates paraquat-induced acute lung injury through inhibition of NF-κB and JNK/p38 MAPK signal pathways. Biomed Res. Int., 2017: 13.
  8. Huang, C. J., Yang, M. C. and Ueng, S. H. (2005): Subacute pulmonary manifestation in a survivor of severe paraquat intoxication. Am. J. Med. Sci.., 330(5): 254-256.
  9. Khodayar, M. J., Kiani, M., Hemmati, A. A., Rezaie, A., Zerafatfard, M. R., Nooshabadi, M. R. R. and Goudarzi, M. (2014): The preventive effect of atorvastatin on paraquat-induced pulmonary fibrosis in the rats. Adv. Pharm. Bull., 4(4): 345.
  10. Kimbrough, R. D. (1974): Toxic effects of the herbicide paraquat.?,?65(4): 65-67.
  11. Lakshman, M., Sudha, V. and Haripriya, B. (2015): Ultrastructural Pathology of Paraquat (PQ) Induced Acute and Subacute Lung Injury in Experimental Rats. International Journal Science and Research (IJSR)., 6(6):1454-1458.
  12. Ma, J., Sun, F., Chen, B., Tu, X., Peng, X., Wen, C., Hu, L. and Wang, X. (2017): Tissue metabolic changes for effects of pirfenidone in rats of acute paraquat poisoning by GC-MS. Toxicol Ind Health., 33(12): 887-900.
  13. Mainwaring, G., Lim, F. L., Antrobus, K., Swain, C., Clapp, M., Kimber, I., Orphanides, G. and Moggs, J. G. (2006): Identification of early molecular pathways affected by paraquat in rat lung. Toxicology., 225(2-3): 157-72.
  14. Neves, F. F., Sousa, R. B., Pazin-Filho, A., Cupo, P., Elias J?nior, J. and Nogueira-Barbosa, M. H. (2010): Severe paraquat poisoning: clinical and radiological findings in a survivor. J Bras Pneumol., 36(4): 513-516.
  15. Novs, R. D., Gon?alves, R. V., Cupertino, M. C., Marques, D. C., Rosa, D. D., Peluzio, M. D. C. G., Neves, C.A. and Leite, J. P. V. (2012): Bark extract of Bathysa cuspidata attenuates extra‐pulmonary acute lung injury induced by paraquat and reduces mortality in rats. Int JExp Pathol., 93(3): 225-233.
  16. Okolonkwo, B. N. and Nwachuku, E. O. (2013): The antioxidant effects of vitamin C on liver enzymes: aspartate aminotransferase, alanine aminotranferease, alkaline phosphatase and gamma-glutamyltransferase activities in rats under Paraquat insult.?Journal of Xenobiotics.,3(1): 24-28.
  17. Okolonkwo, B. N., Nwachuku, E. O., Ene, P. C. and Okeke, C. U. (2014): The preventive effect of vitamin C on the cellular and functional integrity of kidney cells in rats following repeated exposure to paraquat. Journal of Xenobiotics., 4(1): 29-39.
  18. Papiris, S. A., Maniati, M. A., Kyriakidis, V. and Constantopoulos, S. H. (1995): Pulmonary damage due to paraquat poisoning through skin absorption. Respiration., 62(2): 101-103.
  19. Parvez, S. and Raisuddin, S. (2006): Effects of paraquat on the freshwater fish Channa punctata (Bloch): non-enzymatic antioxidants as biomarkers of exposure. Arch. Environ. Contam. Toxicol., 50(3): 392-397.
  20. Pourahmad, M., Jahromi, H. K., Jahromi, Z. K., Sameni, M. H., Abedi, H., Kherameh, Z. K., Mohammadi, S. and Hosseini, M. A. (2016): The protective effect of Aloe vera on paraquat hepatotoxicity. International Journal of Medical Research and Health Sciences., 5(S): 253-258.
  21. Sastry, K. V., Diqui, A. A. and Singh, S. K. (1982): Alterations in some biochemical parameters in the Dnake head fish Channa punctatus exposed clinically to quinalphose.?,?2: 1211.
  22. Shi, X., Zhang, Y. and Wang, Y. (2015): Impact of Xuebijing and ulinastatin as assistance for hemoperfusion in treating acute paraquat poisoning.?Int J ClinExp Med.,8(8): 14018-14023.
  23. Snedecor, G. W. and Cochran, W. G. (1994): Statistical Methods. 8th Edn., Ames: Iowa State Univ. Press Iowa.
  24. Suntres, Z. E. (2002): Role of antioxidants in paraquat toxicity. Toxicology., 180(1): 65-77.
  25. Tavakol, H. S., Farzad, K., Fariba, M., Abdolkarim, C., Hassan, G., Seyed-Mostafa, H. Z. and Akram, R. (2015): Hepatoprotective effect of Matricaria chamomilla. L in paraquat induced rat liver injury. Drug Res.,65(02): 61-64.
  26. Vale, J. A., Meredith, T. J. and Buckley, B. M. (1987): Paraquat poisoning: clinical features and immediate general management.?Human Toxicology.,6(1): 41-47.
  27. Vandenberghe, J. (1995): Hepatotoxicology: mechanisms of liver toxicity and methodological aspects. Toxicology: Principle and Applications., 718.

How to Cite This Article

Kothinti Busa Ashok Kumar Reddy, Mylaram Jeevanalatha, Mekala Lakshman and Matukumalli Usha Rani (2020); AMELIORATIVE ACTIVITY OF ASCORBIC ACID (AA) ON PARAQUAT (PQ) INDUCED SERUM BIOCHEMICAL ALTERATIONS IN EXPERIMENTAL RATS, Int. J. of Adv. Res., 8 (04), 107-112, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/10752

Corresponding Author

Kothinti Busa Ashok Kumar Reddy
Post graduate, Department of Veterinary Pathology, College of Veterinary Science, PVNRTVU, Rajendranagar, Hyderabad-500030, India.
Creative Commons Attribution 4.0 This work is licensed under a Creative Commons Attribution 4.0 International License.