AEROBIC BACTERIOLOGICAL PROFILE OF NEONATAL SEPTICAEMIA AND STUDY OF ESBL PRODUCTION IN GRAM NEGATIVE ISOLATES.

Hundred clinically suspected septicaemic neonates ,admitted in neonatal intensive care unit (NICU) of Government General hospital ,Siddhartha medical college ,vijayawada were studied over a period of one year . Blood culture positivity was 35% , out of which in early onset sepsis, culture positivity was 19(54%) and in late onset sepsis culture positivity was 16(45.7%). out of total isolates gram positive pathogens constituted 20(62.5%), in which coagulase negative staphylococci and staphylococcus aureus were commonest organisms 9 (45%). out of 35 total bacteria isolates , gram negative organisms constitute 15(37.5%). Among the 15 gram negative isolates ,Klebsiella Pneumoniae was commonest (52%),followed by Escherichiacoli 3(20%),Pseudomonas aeruginosa 3(20%) and Acinetobacter 1(8%). Staphylococcus aureus was the commonest organism in gram positive and Klebsiella Pneumoniae is commonest among gram negative bacilli . In late neonatal septicaemia the commonest causative gram negative isolate was Kebsiella Pneumoniae .All gram positive cocci i.e. Staphylococcus Aureus,coagulase negative Staphylococci were 100% sensitive to vancomycin .All gram negative bacilli i.e. Klebsiella Pneumoniae ,Escherichiacoli ,Psuedomonas species and Acinetobacter were 100% sensitive to Imepenem,followed by Cefperazone 80% .Gram negative isolates 15 (75%) were resistant to third generation cephalosporins , and all of them were screened for ESBL production The confirmation of ESBL production was done by double disk synergy test (DDST). out of 8 Klebsiella Pneumoniae isolates screened for ESBL , 5(62.5%) were ESBL producers .out of 3 Escherichia coli , 2(75%) were ESBL producers and 3 pseudomonas species were ESBL producers.In the present study the total number of extended spectrum of beta lactamase (ESBL) production in gram negative bacilli in neonatal septicaemia is 10 (66.6%).