The Possible Protective Effects of Candesartan cilexetil Against Methoteraxte Induced Nephrototoxicity In Rabbits

This study was designed to evaluate the possible protective effects of candesartan cilexetil against methoteraxte induced nephrotoxicity in rabbits . The animal groups were divided into a control group received tap water these animals were anesthetized by ether and then will be sacrificed on day 10. Methoteraxte (MTX) group (Induction group) received (100 mg /kg intraperitonealy a single dose at day 4 to induced nephrotoxicity. The animals were anesthetized by ether & then will be sacrificed on day 10 ,this group served as second positive control of renal damage . Candesartan + Methoteraxte (MTX) group (pre & post-group) in which rabbits treated with Candesartan10 mg /kg orally for 10 days & MTX 100 mg/kg a single dose intraperitonealy at day 4 , the animals were anesthetized by ether and then will be sacrificed on day 10. then estimation of serum levels of urea , creatinine ,albumin and total serum protein (TSP) . Also the kidney tissue homogenate was prepared to evaluate tissue levels of malondialdehyde (MDA) and glutathione (GSH) . Finally, kidney tissue sections were prepared by using paraffin sections technique and stained with hematoxylin and eiosin for histological evaluation .