CHROMOSOMAL ANOMALIES IN INFERTILE COUPLES

Infertility is a major social and multi-factorial sexual fitness conditionresponsibleforaffecting14%ofIndianpopulation in India. Lack of awareness, literacy, inadequate health care facilities, financial problems, etc, are the principal reasons for infertility especially in rural India. The present study describes the chromosomal aberrations associated with reproductive failure. A total of 68 samples, including 4 controls were collected based on sample selection criteria from the North Gujarat region for the present study. Lymphocyte culture was performed in HiKaryo XL RPMI- 1640 for 72 hrs to obtain metaphase stage for karyotyping. GTG banded chromosomes were analyzed by using an automated microscopy system (Carl Zeiss) using Meta Analysis Software. From 68 samples, 63 samples (92.65%) had no chromosomal anomalies. Four samples exhibited heterochoromatin polymorphic variation; 46,XX,9hq+, 46,XY,14ps+ and 46,XX,21ps+, whereas one sample exhibited paracentric inversion 46,XY,inv (8)(p21:q22). Polymorphic variants in chromosomes like 46,XY,14ps+ and 46,XX,21ps+ observed, which are basically pseudo satellites that contain secondary constrictions. The polymorphic variations and structural chromosomal aberration and their association with reproductive failure are discussed in the present studies.