The cytotoxic effect of some chemotheraptic drugs & functional activity of breast cancer patients peripheral blood lymphocytes.

This study has been done on 30 patients presented with breast cancer they visited the Institute and Hospital of Radiotherapy and Nuclear Medicine-Baghdad after mastectomy for chemotherapy. From each patient, 5 ml of peripheral blood was drawn before taking chemotherapy (day 0) with follow up of each patient. After the first chemotherapy dose in about 21 day a second peripheral blood sample was taken, then after a second chemotherapy dose a third sample was taken in day 42 .In each time the peripheral blood lymphocytes were isolated and washed with media and then put it in the culture medium (RPMI) with the drugs methotrexate , vincrestine , adryamicin , 5 Fluorouracil and Endoxan to evaluate the side effects of the drugs on the peripheral blood lymphocytes. Also, we added the mitogen Concanvalin A ( Con A) to compare its effect on lymphocytes with the control groups and then Micro culture Tetrazolium dye assay M.T.T. assay by using ELISA reader on wave length 550 nm. The aqueous solution of the leaves of the vinca rosea was also used for studying the effect on lymphocytes. The results showed the ability of Con A in increasing the cellular activity of peripheral blood lymphocytes. While the aqueous solution of Vinca rosea showed a rapid proliferative effect on Peripheral blood lymphocytes. A control group from 20 healthy women was taken and for each woman 5 ml of peripheral blood was collected and the same step for their blood was processed for comparison with the patient group. The aim of this work is to evaluate the cytotoxic effects of some chemotherapeutic drugs used to treat breast cancer patients on peripheral blood lymphocytes (PBLs). Also to evaluate the effects of the aqueous local extract from Vinca rosea on patients PBLs.