PRODUCTION AND OPTIMIZATION STUDIES OF THE KERATINASE ENZYME BY BACILLUS TEQUILENSIS MBR 25

Feather-degrading bacteria are the enzyme keratinase-degraded community of micro-organisms that may degrade feathers. They are omnipresent in the food industry in feather waste. This food sector produces large quantities of feather waste that can hardly be treated using conventional chemical and physical methods. Due to the peculiar biochemical structure of the feather, mediated by beta-keratin and reinforced by several disulfide bonds, the degradation of feather waste is typically the most cost-effective and environmentally friendly process. The present study identified a medium for the development of keratinase by shaking flask culture (Bacillus tequilensis MBR 25). Feather (1%) and maximum keratinase efficiency have been determined for the highest keratinase output. Keratinase efficiency improved when all parameters were optimized simultaneously.