Analysis of proteins profile and antibacterial activity in haemolymph of Eri silkworm, Samia cynthia ricini after bacterial inoculation

The current study was designed based on proteomic approaches to understand the immune response of Eri silkworm after 24 hour post bacteria inoculation. Analysis of the proteins profile of non inoculated haemolymph, sterile injured haemolymph and bacterial inoculated haemolymph of Eri silkworm after 24 hour post bacterial inoculation by polyacrylamide gel electrophoresis for quantitative and qualitative changes after protein separation. Bacterial inoculation caused haemolymph protein lyses as shown by changes in number of proteins after post inoculation i.e more dominant protein bands were appeared at 60 - 90 KDa and 14 - 20 KDa, as well as specific low density protein or peptide bands at 21-30 KDa and 6-13 KDa were also observed in both gram +ve and gram –ve inoculated insect haemolymph. The antibacterial activity significantly increased in Eri silkworm haemolymph after inoculation with Escherichia coli and Micrococcus luteus. Therefore, we conclude that, this study revealed that the induced to produce antimicrobial substances in the haemolymph of Eri silkworm larvae after the bacterial inoculation and increase the quantity of antimicrobial substances may contribute to the antibacterial activity in haemolymph as an immune response. Finally, we might be concludes that induced antimicrobial substances are play major role insect innate immunity and defence system either destroy or invading, elimination of pathogens of host insect.