Screening of Medium Components for The Enhanced Production of Pseudomonas fluorescens L-Glutaminase by Plackett Burman Design

In this present investigation medium components influencing the production of L-Glutaminase were screened by using Plackett Burman Design. Total 33 different media components comprising of 11 carbon sources, 11 nitrogen sources and 11 mineral sources were screened for enhanced production of L-Glutaminase in submerged fermentation. The statistical analysis viz. regression coefficient and ANNOVA were used to identify the most significant factors among the selected one. A mathematical model was developed to predict the relative influence of different factors. Among 33 different nutrients Gelatin, Tryptone, Starch potato and KCl were found to be most effective media constituents.