DETECTION OF SOME VIRULENCE FACTORS GENES OF Proteus mirablis THAT ISOLATED FROM URINARY TRACT INFECTION

In the present study ,170 urine sample were collect from Patients suffering from urinary tract infection from both sexes and from different ages , who frequents Al-Diwanyia Teaching Hospital, and Al-Diwanyia women and children Hospital in Al-Diwanyia city , the period was from January 2013 to April 2014 to examine Proteus mirablis bacteria. And the results of the facial biochemical tests showed that (30%) (%17.64) sample belong to Proters mirablis . Some of the genes of virulence factors were investigated using Single and Multiplex Polymerase Chain Reaction Technique and they are ureA and ureC responsible for producing urease enzyme , hpmA ,Which is responsible for producing of hemolycin ,zapA Which is responsible for producing protease enzyme , flaA ,Which is responsible for flagella. For the rates of the appearance of these genes were (%96.66) , (%100) , (%100) , (%100) and (%86.66).respectively.