Study of Interleukin-8 Gene Polymorphisms in Egyptian Hepatocellular Carcinoma Patients and Association with Insulin Resistance State

Hepatocellular carcinoma is the six cancer worldwide and the first in Egypt . Interleukin-8 is an angiogenic chemokine with important roles in development and progression of many human malignancies including HCC. This study was designed to investigate the association of IL-8(- 251A/T and +781 C/T) Single-Nucleotide-Polymorphisms with susceptibility of HCC in patients with chronic hepatitis C and to correlate it with insulin resistance. 112 HCV related HCC patients and 105 control subjects were analyzed for serum IL-8, fasting insulin level using (ELISA- technique) and IL-8(- 251A/T and ?+781 C/T) SNPs using PCR-RFLP genotyping analysis, then HCV related HCC patients were subclassified into 79 patients with IR and 33 patients without IR . Results showed that individuals with IL-8 +781 T/T homozygote have lower risk of HCC development (with OR=0.26) while others with C allele have a higher risk (with OR=2.01) and T allele of IL-8 (?+781 C/T) in HCC subjects with and without IR show significant for (AOR) =0.59 while no significant for (OR=0.65). It could be concluded that IL-8(-251 and +?781) SNPs can considered to be a candidate gene for the liability of HCC also, could predict the prognosis of the progression of chronic HCV infection to HCC or not. Moreover, IR and steatosis could be a complicating event of chronic HCV and could be considered as a possible predisposing factor for HCC in such patients inspite of there is found correlation with the studied SNPs in IL-8 gene which need further extended studied to validate these results.