Diagnosis of Brucellosis in Ruminants in Kafr El-Sheikh governorate, Egypt

Brucelosis is one of the most important diseases affecting animals in most of the developing countries. The study aimed to serological detection of Brucellosis in ruminants in Kafr El-Sheikh governorate using RBPT and BPAT as a screening test and ELISA and CFT as a confirmatory test. The obtained result showed that the incidence rate was highest in sheep (12%) followed by cattle (8.4%), goat (6.4%) and finally the lowest incidence rate was in buffaloes (6.1%). The most infected area was Fuwwa and kafr El-sheikh in comparison with other districts. Moreover, iELISA is most sensitive (96.5%) and specific test (98.3%) while BAPT is the lowest sensitive (83.8%) and specific test (90%). The isolated Brucella strain bacteriologically identified and confirmed by PCR as B. meltensis biovar 3.