Isolation and Characterization of Bacillus Subtillus with Potential Production of Nattokinase

The aims of this study were to screened and characterized Bacillus spp. isolate that could produce a natural nattokinase with high activity. The study was carried out on 50 samples collected from different regions in Sudan. Primary screening and characterization of the microorganism showed that five samples (10%) were considered as Bacillus subtilis according to microscopic and biochemical characteristics. Selective medium was prepared for the extraction and production of nattokinase from these new isolates. The selected isolates Bacillus subtilis could produce active nattokinase with inhibition zone diameter ranged from 15-26 mm according to haemolysis and fibrinolytic activity.