IMPACT OF TDZ (THIDIAZURON) PULSE TREATMENT IN SINGLE AND MULTIPLE SHOOT FORMATION IN CALLI OF JATROPHA CURCAS L.

Rather being the widely used green alternative to fossil sources of energy, Jatropha have so far been commercially utilized and has medicinal values too. As the remunerative benefits accruing it is now really important to micropropagate Jatropha in a large extent. Here in a new protocol being proposed to regenerate shoots from calli earlier in time through TDZ pulse treatment. Thidiazuron being a most active cytokinin like substance induces greater in vitro shoot proliferation. Jatopha curcas (CJC - 19) calli when given 3 – 5d TDZ (3mg/L) treatment were capable of giving rise to earlier shoots. Calli obtained from ex vivo leaves was used to study this effect. The distinct shoots were isolated and rooted in MS media containing IBA (0.3 mg/L). The rooted plants were finally transferred to soil in pots.